肺炎对幼鼠心肌细胞内游离钙含量的影响  

Effects of Pneumonia on Myocardial Cells Free Calcium in Infant Rats

作　　者：陈新民[1] 王欢[1] 萧家思[2] 陆建华[2] 叶礼燕[1] 任榕娜[1] 洪新如[1] 

机构地区：[1]南京军区福州总医院儿科 [2]第三军医大学生理教研室

出　　处：《福州总医院学报》1999年第2期6-8,共3页Journal of Fuzhou General Hospital

基　　金：全军"九五"医学科研基金资助课题(98D021)

摘　　要：目的观察肺炎对幼鼠心肌细胞内游离钙离子浓度[Ca^(2+)]_i的影响,探讨肺炎时心肌功能障碍发生的机理.方法采用气管内接种的方法,建立幼鼠金黄色葡萄球菌肺炎模型,分离心肌细胞,运用钙离子荧光指示剂Fura-2/AM,检测心肌细胞内[Ca^(2+)]浓度.结果对照组24h(116.3±15.8)72h(120.5±12.6)和120h(118.2±13.5)相比,肺炎组接种后24h(138.7±13.6)(P<0.05)、72h(161.0±15.2)(P<0.01)和120h(173.6±11.2)(P<0.01)心肌细胞内游离钙含量升高.结论肺炎可引起幼鼠心肌细胞内钙超载,心肌细胞内钙稳态的变化可能是肺炎影响心肌功能的重要病理生理基础之一.Objective To elucidate the effects of pnuemonia on the myocardial cells free calcium and its mechanisms. Methods A peumonia mode has been made with staphylococcus. The myocardial cells was isolated by collagenase. the free calcium contents of myocardial cells were measured with fluorescein fura -2/AM at 24h(116.3±15.8), 72h(120.5±12.6), 120h(118.2±13.5) of inoculation. Results Compared to control group, the free calcium contents of myocardial cells increased at 24h(138.7±13.6) (p<0.05), 72h(161.9±15.2)(p<0.01),120h(173.6±11.2)(p<0.01) in the pneumonia group rats. Conculation The changes of free calcium contents of myocardial cells may be one of the important biochemical indicators for cardiae function alterations associated with pneumonia.

关 键 词：心肌细胞 肺炎 幼鼠 胞内游离钙 心肌功能 钙含量 影响 Ca^2+ 钙稳态 病理生理基础 

分 类 号：R542[医药卫生—心血管疾病] R563.1[医药卫生—内科学]