不同浓度不同氮源对小麦离体叶谷氨酰胺合成酶及其相关酶的影响  

Influence of Difference Nitrogen Sources at Different Concentrations On the Activity of Glutamine Synthetase and the Reletive Enzymes of Nitrogen Metabolism in Wheat Separated Leaves

作　　者：印莉萍[1] 刘祥林[1] 柴小清[1] 李蓉[1] 洪剑明[1] 左文倩 邱泽生[1] 

机构地区：[1]首都师范大学生物系

出　　处：《首都师范大学学报（自然科学版）》1997年第2期95-99,共5页Journal of Capital Normal University：Natural Science Edition

摘　　要：利用酶活测定和Northern分子杂交等技术,研究了小麦幼苗离体叶片在不同浓度的不同氮源供应下,其谷氨酰胺合成酶(GS)、天冬酰胺合成酶(AS)、硝酸还原酶(NR)以及GS基因在转录水平GS-mRNA的变化。结果表明:NH_4^+处理的小麦,其叶部GS活性比NO_3~_处理的高,Northern杂交结果说明GS-mRNA转录量与GS活性一致;NO_3~_可激活NR活性,对AS有较明显的诱导作用。Influence of different nitrogen sources at different concentrations on the activity of glu-tamin synthetase(GS) and the relative enzymes of nitrogen metabolism in wheat separated leaves have been studied by enzymes activity assay and northern blot. The results indicated that the glutamine synthetase (GS) was promoted stronger in leaves of wheat by NH4+-N than by NO3-N. GS-m RNA of northern blot is consistent with GS activity. NO3- induced apperently the activity of NR and asparagine synthetase

关 键 词：小麦幼苗 氮源 谷氨酰胺合成酶 浓度 离体叶片 硝酸还原酶 杂交 GS 相关酶 活性 

分 类 号：S512.1[农业科学—作物学]