体外消化试验法的初步评价  

A Preliminary Appraisal of The In Vitro Digestion Method

作　　者：陈化鹏[1] 李枫[1] 孙中武[1] 马建章[1] 王槐[1] 李飞[1] 

机构地区：[1]东北林业大学

出　　处：《东北林业大学学报》1997年第5期81-84,共4页Journal of Northeast Forestry University

基　　金：国家自然科学基金

摘　　要：研究旨在评价３个因素（发酵器类型、胃蛋白酶溶液的浓度、瘤胃液的来源）对５类饲料植物干物质体外消化率测定结果的影响。结果表明，发酵器类型（恒温水浴箱和恒温保温箱）和胃蛋白酶溶液的浓度（２．３％和４．６％），对５类饲料植物干物质体外消化率的测定结果均无显著的影响。同样，不同来源的瘤胃液（家羊、马鹿、狍）对饲料植物干物质体外消化率的测定值也无显著的影响。In this study, we evaluated the influence of three factors (the type of incubator, the concentration of pepsin solution and inoculum sources) on the accuracn of the in vitro digestion method using five types of forages (twigs and leaves of aspen, sedges, forbs, and fems) in trials. The results showed that there was no significant difference in in vitro dry matter digestibilities (IVDMD) of five types of forages when the water bath and the drying oven were used as incubators or two kinds of concentrations (2.3% and 4.6%) of the pepsin solution were used in the trials. No significant difference was found either, in IVDMD of five types of forages when inocula were taken from domestic sheep, wapiti(Cervus elaphus) and roe deer (Capreolus capreolus), respectively. It is sugested that inocula from domestic sheep, instead from wapiti and roe deer can be used in vitro digestion trials for measuring IVDMD of forages used by wapiti and roe deer, especially in some cases where emphasis is put on comparing and assessing the relative nutritional values of forages used by wild ruminant species like wapiti and roe deer.

关 键 词：体外消化试验 马鹿 狍 

分 类 号：S816.15[农业科学—饲料科学]