大黄对烫伤大鼠肠粘膜上皮细胞肿瘤坏死因子受体基因表达的影响  

Effects of rhubarb on expression of tumor necrosis factor receptor in intestinal epithelium after thermal injury

作　　者：李红江[1] 陈德昌[1] 

机构地区：[1]第二军医大学长征医院急救科,上海200003

出　　处：《中国急救医学》2001年第1期1-3,共3页Chinese Journal of Critical Care Medicine

基　　金：国家自然基金资助项目 (No.39570688)

摘　　要：目的　观察烫伤和内毒素二次打击后肠粘膜上皮细胞表达肿瘤坏死因子受体TNFR1和TNFR2 的情况 ;研究大黄对肠粘膜上皮细胞内TNFR1、TNFR2 基因表达的影响。方法　应用随机、前瞻性研究方法。采用大鼠烫伤和内毒素二次打击模型。应用原位杂交法检测肠粘膜上皮细胞TNFR1和TNFR2 的基因表达情况 ,以及血浆内TNF -α浓度。结果　原位杂交显示烫伤和内毒素二次打击后肠粘膜上皮细胞内TNFR大量表达 ,以TNFR1为主 ,TNFR2 仅小量表达 ,大黄 ( 50mg/kg)治疗后两种受体表达均明显减少。烫伤和内毒素二次打击后血浆内TNF -α浓度明显增高。大黄治疗后TNF -α浓度明显降低 (P<0 0 1)。结论　大黄可抑制病理情况下TNFR1、TNFR2 表达 ,降低血浆内TNF -α水平 ,抑制TNFObjectives ①To study the effects of thermal injury and two-hit, induced by endotoxin, on the expression of tumor necrosis factor receptors(TNFR 1 and TNFR 2) in intestinal epithelial cells;②To test the hypothesis that rhubarb can inhibit the expression of TNFR 1 and TNFR 2 in systemic inflammatory response syndrome induced by thermal injury and the two-hit. Methods Prospective and randomized trial. The rat burn model was used. Endotoxin at a dose of 20 mg/kg was continuously infused 12 hours after burn in remaining 12 hours. Hybridization in situ was applied to evaluate the expression of TNFR 1 and TNFR 2 in intestinal epithelial cells. The concentration of TNF-α in plasma was also observed. Results Hybridization in situ revealed that thermal injury and two-hit induced by endotoxin could obviously increase the expression of TNFR 1 and TNFR 2 in intestinal epithelial cells. However, the TNFR 1 was prevailing. The rhubarb could down-regulate both of the receptors. There was very high concentration of TNF-α in plasma after thermal injury and the two-hit. Rhubarb evidently lowered the concentration of TNF-α in plasma ( P <0.01). Conclusion Rhubarb could down-regulate the expression of TNFR 1 and TNFR 2 in intestinal epithelial cells after thermal injury and the two-hit. It also decreased the concentration of TNF-α in plasma. [

关 键 词：大黄 烧伤 内毒素 肿瘤坏死因子 受体 

分 类 号：R644[医药卫生—外科学]