包核为H_2O_2的超声造影剂研究  

Study On New Ultrasonic Contrast Agent Encapsulating H_2O_2

作　　者：卓仁禧[1] 丁雄军[1] 傅功成[1] 李威[1] 

机构地区：[1]教育部生物医用高分子材料重点实验室

出　　处：《武汉大学学报（理学版）》2000年第6期645-648,共4页Journal of Wuhan University:Natural Science Edition

摘　　要：以双氧水为包核 ,以卵磷脂 ( PC) ,二肉豆蔻酰磷脂酰胆碱 ( DPPC) ,司盘 6 0 ( Span 6 0 ) ,吐温 6 0 ( Tween6 0 )等为膜材 ,用逆相蒸发法或超声法制备了 12种囊泡 ,其平均直径在 0 .1～ 3μm范围内 .研究了囊泡的包封率、形状以及尺寸大小随时间变化的情况 .实验结果表明采用逆相蒸发法制得的囊泡稳定性较好 ;而膜材不同 ,囊泡的稳定性也有明显的差别 .选取包封率较高的一种囊泡 ,在 37℃ ,过氧化氢酶存在下测定了 H2 O2 的释放速率 .将部分囊泡通过静脉注射到家兔体内 。New microbubbles were prepared through two different methods by the use of H 2O 2 as the nuclei and PC,DPPC,Span 60 or Tween 60 as the encapsulating membrane. The size of the microbubbles ranged from 0.1 μm to 3 μm and encapsulating effectiveness reached 25% to 42%. nio2, which was prepared from Tween 60 exhibited better stability than that of the other microbubbles. Releasing rate of H 2O 2 from some of the microbubbles were determined at 37℃ in the presence of hydrogen peroxidase. The animal experiment results of ultrasonic imaging showed that some of the microbubbles could produce hepatic enhancement due to back scattering of O 2 which stemmed from enzymatic catalysis of H 2O 2.

关 键 词：超声造影剂 囊泡 过氧化氢 背向散射 

分 类 号：O631[理学—高分子化学]