组织工程动脉血管平滑肌种子细胞的构建  

Construction of vascular smooth muscle seed cells for artery tissue engineering

作　　者：刘庆阳[1] 谷涌泉[2] 罗涛[2] 郭连瑞[2] 吴英锋[2] 卢光[2] 孙海晨[3] 宋业光[4] 

机构地区：[1]首都医科大学宣武医院,血管外科研究所,北京市100005 [2]首都医科大学宣武医院血管外科,北京市100053 [3]首都医科大学宣武医院外科实验室,北京市100053 [4]中日友好医院整形外科,北京市100029

出　　处：《中国临床康复》2005年第3期138-139,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:为组织工程动脉提供种子细胞,从而构建出组织工程化动脉。方法:实验于2004-03/05在首都医科大学宣武医院外科实验室完成。采用组织贴块法培养成年狗主动脉平滑肌细胞,并进行细胞传代、鉴定及形态学观察。结果:通过严格取材即可获得高纯度培养的平滑肌细胞,其形态学特征明显,但随培养代次的增加,其增殖能力及形态出现改变。结论:成年狗主动脉平滑肌细胞可作为动脉组织工程的种子细胞,其增殖能力有待于进一步加强。AIM:To provide seed cells for artery tissue engineering to construct tissue engineered artery. METHODS:The experiment was conducted in the Laboratory for Surgery of Xuanwu Hospital,Capital University of Medical Sciences from March to May 2004.The smooth muscle cells were isolated from adult canine aortas by tissue section adherence methods followed by subculture,cell passage,identification and morphological observation. RESULTS:The smooth muscle cells inahigh purity could be obtained by strict operation.Morphological characteristics of the smooth muscle cells are remarkable.But the ability of proliferation and modality of the smooth muscle cells changed with the more cultured passages. CONCLUSION:The smooth muscle cells of adult canine aortas can served as seed cells for artery tissue engineering.The ability of proliferation of smooth muscle cells should be enhanced further.

关 键 词：生物医学工程 细胞培养 肌细胞 平滑肌 

分 类 号：R318[医药卫生—生物医学工程]