大花蕙兰不同基因型组培繁殖系数的差异性  

Differences of Cymbidium hybridum Propagation Ratio in Different Genotype

作　　者：李杰[1] 黄敏仁[1] 王明庥[1] 蔡汝 匡鹏安 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]江苏省兰花工程研究中心,江苏泰州225321 [3]四川省巴中市巴州区林业局,四川巴中636000

出　　处：《南京林业大学学报（自然科学版）》2005年第1期98-100,共3页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省科技攻关计划资助项目(BE2003384)

摘　　要：研究了16种基因型大花蕙兰芽增殖快繁繁殖系数的差异性。结果表明:(1)在给定的同一培养基中,16种基因型的平均繁殖系数为2.33,繁殖系数低于2.10以下的观测值占总数的 50%,相同培养基配方在多品种种苗生产中不能应用;(2)繁殖系数的方差分析和多重比较显示,差异的显著性在不同基因型间达不同水平,离散性和集中性具有分组特性,可以用适度的配方方案探讨分组规律;(3)影响芽增殖繁殖系数差异的主要因子是细胞分裂素的浓度水平,二者关系可用二次式模型拟合,拟合的模型可应用于大花蕙兰的分组快繁。Differences of sixteen cultivated Cymbidium hybridum orchids in bud propagation ratio were studied.The results are as follows:(1)In the same proliferation medium,the means of propagation ratio is 2.33 and the values below 2.10 are as much as 50%,so it can not use the same medium to proliferate in the procedure of different genotype plantlets production;(2)Analyses of variance and multiple range tests show that the differences of propagation ratios in different genotype are significant or extremely significant,but different means also tend towards cluster and scatter,we can select adaptive medium to probe this rule.((3)The) main factors that influence bud proliferation are the concentration of cytokinins,and the relations can be formulated by conic models.By using those models,good results can be obtained in the practice of C.hybridum plantlet proliferation.

关 键 词：大花蕙兰 基因型 繁殖系数 差异性 

分 类 号：S682.31[农业科学—观赏园艺]