淫羊藿对大鼠内脏器官PDC钙通道及其心肌缺血性损伤的影响  

Effect of Herba Epimedii on the Visceral Organs and Myocardial Ischemia Injury in Rats

作　　者：杨远友[1] 刘宁[1] 莫正纪[2] 谢建平[1] 廖家莉[1] 莫尚武[1] 

机构地区：[1]四川大学原子核科学技术研究所辐射物理与技术教育部重点实验室,成都6100642 [2]四川大学华西药学院,成都610041

出　　处：《四川大学学报（自然科学版）》2005年第1期122-127,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(10175044)

摘　　要：目的:研究淫羊藿不同提取方法对大鼠主要内脏器官45Ca内流、外溢的作用规律及对大鼠心肌缺血性损伤的影响.方法:用45Ca跨膜测量技术研究淫羊藿不同提取物对大鼠心脏、动脉平滑肌、肝、肾脏等4种器官电压依赖钙通道(PDC)的作用特性;用大孔树脂分离、鉴定淫羊藿的有效成分.结果:1.淫羊藿对心脏、动脉平滑肌、肾脏等3种器官45Ca跨膜流动的影响作用极显著,既能阻滞45Ca大量内流,又能促进已流入细胞中的45Ca外溢;2.淫羊藿的碱提组对内脏器官钙通道的调控作用优于醇提组和水提组.3.淫羊藿提取物对药物和结扎冠状动脉所致大鼠心肌缺血性损伤均有保护作用.结论:淫羊藿对多种内脏器官钙通道有较强的调控能力,对维持细胞内Ca2+浓度的稳定和细胞的正常生理功能具有重要意义;淫羊藿对大鼠心肌缺血性损伤具有明显的保护作用.Objective: study the influencing rules about ()^(45)Ca influx and efflux of rat's principal visceral organs with the different extracts; study the effect of Herba Epimedil's extracts on myocardial ischemia injury in rats. Method: 1.wide aperture resin were used to isolate chemical components; 2. ()^(45)Ca transmembrane influx technique was used to study influencing rules and substance foundation. Results: 1.the extracts of Herba　Epimedii have significant effects on PDC ()^(45)Ca channel of three visceral organs in rats: heart, artery, smooth muscle and kidney. They not only block ()^(45)Ca coming into cells, but also can facilitate efflux of ()^(45)Ca in the cell. 2. the regulating effect about the extracts by alkali is better than those by alcohol and water. 3. Herba Epimediis 　extracts possess significant protection effects on myocardial ischemia induced either by drugs or by ligating the coronary artery. Conclusion: these results suggested that Herba Epimedii had significant regulating effect on PDC ()^(45)Ca channel, that Herba Epimedii possesses significant protection effects on myocardial ischemia.

关 键 词：淫羊藿 ^45CA 电压依赖钙通道(PDC) 心肌缺血 

分 类 号：TL99[核科学技术—核技术及应用] Q4[生物学—生理学]