不同温度和激素浓度对春兰部分生理活动的影响  

Physiological Reactions of Cultured Cymbidium goeringii to Different Temperatures and Concentrations of Cytokinin

作　　者：文璨[1] 傅华龙[1] 卿人韦[1] 兰利琼[1] 张露洁[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2005年第1期171-174,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：　通过改变温度,细胞分裂素6 BA浓度后对春兰的过氧化物酶活性、丙二醛、可溶性蛋白质、可溶性多糖含量变化的测定,研究不同培养条件对春兰生理活动的影响.结果显示:春兰的过氧化物酶活性受温度及细胞分裂素浓度影响的强弱顺序是18℃、30℃、26℃、22℃,8mg/L、2mg/L、4mg/L、6mg/L;丙二醛含量受温度及细胞分裂素浓度影响的强弱顺序是30℃、22℃、18℃、26℃,2mg/L、8mg/L、4mg/L、6mg/L;可溶性蛋白质含量受温度及细胞分裂素浓度影响的强弱顺序是26℃、30℃、22℃、18℃,6mg/L、4mg/L、8mg/L、2mg/L;可溶性多糖含量受温度及细胞分裂素浓度影响的强弱顺序是26℃、22℃、30℃、18℃,6mg/L、4mg/L、8mg/L、2mg/L.由此表明春兰在本实验温度范围内,最适生长温度为26℃,最适细胞分裂素6 BA浓度为6mg/L.To identify the suitable range of temperature and cytokinin of Cymbidium goeringii, series of culture conditions were designed with the cross of different temperature and cytokinin (6-BA ) (18℃, 22℃, 26℃, 30℃; 2mg/L, 4mg/L, 6mg/L, 8mg/L). The activities of peroxidase (POD), the contents of malonaldehyde (MDA), dissolved protein and total sugar were determined by TU-1800 and other apparatuses. And I got the results below. The activities of POD show 18℃、30℃、26℃、22℃, 8mg/L、2mg/L、4mg/L、6mg/L; The content of MDA show 30℃、22℃、18℃、26℃, 2mg/L、8 mg/L、4mg/L、6mg/L; The content of dissolved protein show 26℃、30℃、22℃、18℃, 6mg/L、8mg/L、4mg/L、2mg/L; The content of dissolved total sugar show 26℃、22℃、30℃、18℃, 6mg/L、4mg/L、8mg/L、2mg/L. According to the data above.It was known that the most suitable temperature and cytokinin are 26℃ and 6mg/L.

关 键 词：春兰(Cymbidium goeringii) 温度 细胞分裂素6-BA 

分 类 号：Q945[生物学—植物学]