四磺基铁(Ⅲ)酞菁二级散射和反二级散射法测定人血清白蛋白  

Double Scattering and Antidouble Scattering Spectra for the Determination of Human Serum Albumin with Tetrasulponaeted Ferric Phthalocyanine

作　　者：高建华[1] 李华岑[1] 杨潞[1] 王俊臣[1] 龚雪云[1] 

机构地区：[1]郑州大学化学系,郑州450052

出　　处：《分析科学学报》2005年第1期84-86,共3页Journal of Analytical Science

摘　　要：在pH2.0的Clark Lubs缓冲液中,人血清白蛋白与四磺基铁(Ⅲ)酞菁结合而使二级散射和反二级散射急剧增强。在0～2.3mg/L和0～3.4mg/L浓度范围内,二级散射和反二级散射的增强与人血清白蛋白浓度呈线性关系,检出限分别为0.40mg/L和0.16mg/L。还考察了某些共存物质的影响,讨论了反应机理。In a Clark-Lubs buffer at pH 2.0, human serum albumin (HSA) can bind with tetrasulponaeted ferric phthalocyanine to form new products, resulting in the great enhancement of second-order scattering(SOS) and anti second-order scattering(ASOS) spectra. The enhanced intensities of SOS and ASOS are proportional to the concentration of HSA in the range of 0～2.3 mg/L and 0～(3.4) mg/L with the detection limitis of 0.40 mg/L and 0.16 mg/L,respectively. The influence of some coexisting substance is investigated and the interaction mechanism of protein and dyes is discussed.

关 键 词：四磺基铁(Ⅲ)酞菁 二级散射 反二级散射 人血清白蛋白 

分 类 号：O657.39[理学—分析化学] Q51[理学—化学]