醒脑通脉胶囊对脑缺血再灌注损伤神经细胞凋亡的影响  

Effect of Xingnao Tongmai Capsule on nerve cell apoptosis in rats with cerebral ischemia-reperfusion injury

作　　者：刘泰[1] 韦必清[2] 韦玉进[3] 

机构地区：[1]广西中医学院第一附属医院神经内科,广西南宁530023 [2]广西中医学院,硕士研究生广西南宁530001 [3]广西中医学院第一附属医院综合实验室,广西南宁530023

出　　处：《辽宁中医杂志》2005年第2期89-91,共3页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的 :观察醒脑通脉胶囊对大鼠脑缺血再灌注损伤神经细胞凋亡的影响 ,探讨其神经保护作用机制。方法 :采用双侧颈总动脉结扎法建立大鼠脑缺血再灌注模型 ,2 4只大鼠随机分为正常对照组、缺血再灌注模型组、醒脑通脉胶囊组、尼莫地平组 ,每组 6只。采用Hoechst染色法及荧光显微镜观察大脑皮质细胞凋亡情况。结果 :脑缺血再灌注模型组大鼠在脑缺血再灌注 2 4h后大脑皮层神经细胞凋亡百分率升高 (P <0 0 1) ,醒脑通脉胶囊能降低大脑皮质神经细胞百分率 ,与脑缺血再灌注模型组比较 ,差异有显著性 (P <0 0 5 )。结论 :醒脑通脉胶囊能减轻脑缺血再灌注后大脑皮质神经细胞凋亡 。Objective:To study the effects on nerve cell apoptosis in rats with cerebral ischemia-reperfusion injury and the nerve protecting mechanism of Xingao Tongmai Capsule.Methods:The rats model was established by bilateral common carotid artery blocking,24 rats were divided randomly into the normal control group,cerebral ischemia-reperfusion group,Cingnao Tongmai Capsule treatmen group,nimodipine treatmen group.hoechst staining was used and the apoptosis of cerbral cortex was observed by fluorescence microscope.Results:The cerbral cortex apoptotic rate was increased significantly in the cerebral ischemia-reperfusion group than those in ghe normal control group after 24 hhours of operation (P<0.01),Xingnao Tongmai Capsule could lower the cerbral cortex apoptotic rate significantly,cimpared with those in the cerbral ischemia-reperfusion group (P<0.05).Conclusion:Xingnao Tongmai Capsule treatme-reperfusion injury,that would be one of the nerve protecting mechanism.

关 键 词：醒脑通脉胶囊 缺血再灌注 神经细胞 凋亡 实验研究 中医药疗法 

分 类 号：R285.5[医药卫生—中药学]