软脉降脂胶囊对载脂蛋白E基因敲除小鼠动脉粥样硬化影响的实验研究  

Preliminary Research of Ruanmaijiangzhi capsule’s Effect in Preventing the Arteriosclerosis of Apolipoprotein E-knockout Mice

作　　者：马慧萍[1] 郑广娟[2] 张静[2] 赵洪敏[2] 

机构地区：[1]山东中医药大学中西医结合基础专业2002级 [2]山东中医药大学

出　　处：《中西医结合心脑血管病杂志》2005年第2期132-134,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：山东省自然基金课题 (No.Y2 0 0 1C1 4 )

摘　　要：目的　观察软脉降脂胶囊对载脂蛋白E基因敲除载脂蛋白E基因敲除小鼠 (ApoE)高脂血症及动脉粥样硬化(atherosclerosis ,AS)的影响。方法　 6周龄ApoE小鼠 ,随机分为高脂血症组与软脉降脂胶囊大、中、小剂量组 ,相同遗传背景的同龄正常C57BL/ 6J小鼠作为正常对照组。软脉降脂胶囊组灌服其生药 ,高脂血症组、正常对照组均灌服生理盐水。连续灌胃 2 4周后 ,下腔静脉取血测血脂 ;取主动脉做形态学观察及图像分析。结果　高脂血症组血清总胆固醇 (TC)、三酰甘油 (TG)、低密度脂蛋白(LDL -C )明显高于正常对照组 (P <0 .0 5 ) ,软脉降脂胶囊组明显低于高脂血症组 (P <0 .0 5 ) ;图像分析测量结果显示高脂血症组AS病变显著 ,与正常对照组比较斑块总面积明显增加 (P <0 .0 1) ;软脉降脂胶囊组AS病变较高脂血症组明显减轻 ,斑块总面积明显减小(P <0 .0 1)。结论　软脉降脂胶囊可降低ApoE小鼠血清TC、TG、LDL -C含量 ,有对抗动脉粥样硬化斑块形成的作用。Objective To observe the effect of Ruanmaijiangzhi capsule's (RMJZ) in atherosclerosis(AS) of apolipoprotein E-knockout [ApoE(-/-)]mice.Methods Six-week old ApoE(-/-) mice were divided randomly into four groups:Hyperlipidimia control group(fed with saline),large dose RMJZ group,medium dose RMJZ group and small dose RMJZ group.Six-week old heathy mice (C 57BL/6J mice ) were the normal control group (fed with saline ). At week 24, the serum lipid TC, TG, LDL-C and HDL-C) were measured, and the aortas were preceded in the formalin for morphological research and photograph analysis.Results TC,TG and LDL-C of Hyperlipidimia control group were significantly increased compared with normal control group(P<0.05 );TC,TG and LDL-C of RMJZ group were definitely lower than those of Hyperlipidimia control group(P<0.05).The results of pathomorphology suggested that RMJZ can decrease the area of aortic lipid plaque and reduced the pathological changes and the degree on hyperlipidemia.Conclusions RMJZ can lower the serum cholesterol and relieve the AS lesions of the ApoE(-/-) mice.

关 键 词：降脂胶囊 高脂血症 小鼠 对照组 正常 载脂蛋白E基因 动脉粥样硬化 LDL—C TG 敲除 

分 类 号：R285[医药卫生—中药学]