不同培养方式中肺腺癌细胞系A549表达E-钙粘素的差异及其意义  

Differences in E-cadherin expression of lung adenocarcinoma cell line A549 under different culture conditions and the related significance

作　　者：黄海辉[1] 胡绍毅[1] 边志衡[1] 何建明[1] 梁后杰[1] 

机构地区：[1]第三军医大学西南医院肿瘤科,重庆400038

出　　处：《第三军医大学学报》2004年第24期2185-2187,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 1710 6 7)~~

摘　　要：目的　初步探讨E 钙粘素在肿瘤群集耐药中的可能机制。方法　采用旋转培养瓶结合台式水浴恒温振荡器旋转培养的方法 ,进行肺腺癌细胞A5 49体外立体培养 ,同时进行普通平面培养 ,通过流式细胞技术、Westernblot、原位细胞凋亡检测试剂盒、四唑盐 (MTT)比色法及血球计数器计数法检测细胞周期 ,E 钙粘素的表达 ,细胞凋亡率及对阿霉素(ADM)敏感性。结果　①立体培养细胞存在E 钙粘素的高表达 ;②同平面培养比较 ,立体培养细胞出现细胞周期G1 期阻滞、细胞凋亡率低、对ADM敏感性低等表现 ,给予E 钙粘素阻滞剂SHE78 7后 ,两者差异明显缩小。结论　E 钙粘素介导的细胞 细胞粘附作用可通过调控细胞周期以及细胞凋亡 ,影响肺腺癌A5 49细胞对ADM的敏感性 ,在肿瘤群集耐药中发挥作用。Objective To investigate the roles of E-cadherin on multicellular resistance of lung adenocarcinoma cell line A549. Methods Three dimensional culture of A549 cells was achieved using rotatable culture bottle and desk-top water bath shake. Flow cytometry, Western blot, in situ cell apoptosis detecting kit, MTT, and blood cell counter were employed to detect the expression of E-cadherin, cell apoptosis, and susceptibility to ADM of A549 cells under different culture conditions. Results Compared with cells of two dimensional culture system (2D system), higher E-cadherin expression was observed in cells of three dimensional culture system (3D system), and G 1 phase arrest, lower cell apoptosis rate, and decreased susceptibility to ADM were found in cells of 3D system. The differences diminished significantly after using SHE78-7 as the blocker of E-cadherin. Conclusion E-cadherin-mediated enhancement of intercellular adherence can function in multicellular resistance of lung adenocarcinoma A549 cells through influencing cell cycle, apoptosis, and susceptibility to ADM.

关 键 词：肺腺癌细胞系 A549 E-钙粘素 细胞周期 细胞凋亡 群集耐药 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R730.2[医药卫生—基础医学]