玉米细菌性枯萎病及其病原菌的检测技术  

Bacterial Wilt of Maize and the Detection of Pantoea stewartii subsp. stewartii

作　　者：吴琼[1] 陈枝楠[2] 范怀忠[1] 金显忠[2] 

机构地区：[1]华南农业大学资源与环境学院,广州510640 [2]深圳出入境检验检疫局,深圳518010

出　　处：《中国生物工程杂志》2004年第12期22-25,31,共5页China Biotechnology

摘　　要：玉米细菌性枯萎病是影响玉米生产的重要病害 ,该病害通过带菌的种子进行远距离传播 ,对该病菌的检测成为防止和控制该病害的重要手段。介绍现有的检测该病菌的各种方法 ,即黑色素选择培养法、double sandwichELISA以及RAPD PCR ,LCR PCR ,Nested PCR ,multiplePCR和荧光实时PCR等分子生物学检测方法 ,并对这些方法的特性进行比较和探讨。Pantoea stewartii subsp. stewartii is the causative agent of bacterial wilt of corn, and is responsible for severe economic losses. The disease can be seed transmitted at low frequencies. This article focused on the detection methods of this pathogen. Several methods of detection were introduced as nigrosine selective medium, double-sandwich ELISA and various PCR based assays. The Differences of previously reported PCR assays were presented in details. The advantages and disadvantages of each method were discussed respectively.

关 键 词：分子生物学检测 实时PCR 培养法 带菌 病原菌 ELISA 病菌 细菌性枯萎病 病害 玉米 

分 类 号：Q949.32[生物学—植物学] S436[农业科学—农业昆虫与害虫防治]