体外培养的牛乳腺上皮细胞形态研究  

Study on the appearance of vitro-cultured bovine mammary epithelial cells

作　　者：彭新荣[1] 郑月茂[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2005年第1期13-17,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"高技术研究发展计划项目(2001AA213081)

摘　　要：　通过有效的细胞培养方法获得了牛乳腺上皮细胞系,并系统观察了乳腺上皮细胞的长出、贴壁、聚集、迁移、分裂、分化、凋亡等一系列形态变化。结果发现,原代培养的乳腺上皮细胞大多数呈卵圆形,细胞之间连接成片,单层生长,如鹅卵石铺过路面,乳腺上皮细胞和成纤维细胞混生时分区生长,界线明显。传代的乳腺上皮细胞呈岛屿状聚集生长,细胞核呈圆形或椭圆形,核仁2～4枚;在含雌激素的培养液中,细胞出现双核或多核现象,但仍表现一定的接触抑制现象。多次传代后的乳腺上皮细胞含不同的细胞类型,通过光镜观察,部分细胞仍保持较快的分裂增殖能力;部分细胞渐渐分化,出现长形细胞、三角形细胞。上皮细胞增殖分化可形成圆顶型结构,乳腺上皮细胞可产生并分泌乳汁,分泌到细胞外的乳汁流动形成网状结构。Bovine mammary epithelial cell lines were obtained by culturing tissue fragment.Then the appearance of these cells were studied by light microscope.The results are as follows:The fibroblast cell and the bovine mammary epithelial cells grow in different areas.The bovine mammary epithelial cells were cultured in vitro through single-cell afloat,which formed island monolayer aggregates,then the cells propagated and formed dome-like construction.The cell nucleus is circular or oval with 2-4 cores.In the culture medium with estrogen,the nucleus type changed.The mammary epithelial cells can be developed into many cell types,the majority of which are short shuttle-like or guboidal.The cells connected with each other properly and formed net-like construction.

关 键 词：体外培养 牛 乳腺上皮细胞 细胞形态 

分 类 号：Q813.11[生物学—生物工程]