人胎盘TRAIL基因的cDNA克隆及序列测定  

Cloning and sequencing of cDNA of TRAIL from human placenta

作　　者：张明烽[1] 唐爽[1] 宋红卫[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2005年第2期6-8,13,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"高技术资助项目(2001AA213081)

摘　　要：　根据GeneBank(U37518)中TRAIL(肿瘤坏死因子相关的凋亡诱导配体)的cDNA序列,设计扩增引物。采用RT-PCR从人胎盘中扩增出TRAIL基因的全长cDNA。产物纯化后连至pGEM-TEasy载体,转化大肠杆菌DH5α。筛选阳性克隆菌,酶切鉴定并进行序列测定。结果表明,本试验成功地克隆了TRAIL基因的1039bp全长cDNA。According to the cDNA sequence of TRAIL (TNF-related apoptosis-inducing ligand,TRAIL) derived from the Genebank (U37518),a pair of primers were designed.RT-PCR was applied to amplify the whole cDNA of TRAIL gene.The DNA fragment was cloned into pGEM-T Easy vector and transformed into E.coli DH5α host bacteria.NotⅠ endonuclease digestion and sequencing of selected positive cloned bacteria were conducted.Result showed the whole 1 039 bp cDNA of TRAIL was successfully cloned.

关 键 词：肿瘤坏死因子相关的凋亡诱导配体(TRAIL) RT-PCR 序列测定 人胎盘 

分 类 号：Q786[生物学—分子生物学]