褐藻小粘膜藻的化学成分  

CHEMICAL CONSTITUENTS OF BROWN ALGA LEATHESIA NANA

作　　者：徐秀丽[1] 范晓[1] 宋福行[1] 杨永春[2] 韩丽君[1] 石建功[2] 

机构地区：[1]中国科学院海洋研究所 [2]中国医学科学院暨中国协和医科大学药物研究所,北京100050

出　　处：《海洋与湖沼》2005年第1期18-23,共6页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学重点基金资助项目;29932030号;国家"863"高技术发展计划专项;2001AA620403号。

摘　　要：应用正相硅胶、生物胶BioBeads、凝胶SephadexLH20柱色谱和反相HPLC等层析方法,从小粘膜藻(Leathesianana)中分离得到7个化合物,通过IR、MS、1D和2DNMR等波谱技术鉴定为4羟基苯甲酸(1)、3羟乙酰基吲哚(2)、2,3二溴4,5二羟基苯甲醛(3)、3溴4,5二羟基苯甲醛(4)、2,3二溴4,5二羟基苯甲基乙醚(5)、双(2,3二溴4,5二羟基卞基)醚(6)和3溴4(2,3二溴4,5二羟基苯甲基)5甲氧甲基苯二酚(7)。Leathesia nana is a brown alga of Leathesiaceae family and widely distributed in gulf of the Yellow Sea, China. The ethanolic extract of Leathesia nana showed selective cytotoxicity in our in vitro biological screening against several human cancer cell lines, including KB and HT-29 cell lines with LD_~50 12.65 and 40.60 μg/ml, respectively. It showed indistinct cytotoxicity to normal cell NIH-3T3 with LD_~50 >50 μg/ml. Thin layer chromatography indicated the presence of compounds positive to ferric chloride spray reagent. In order to searching for new bioactive lead compounds, we investigated the chemical constituents of the brown alga Leathesia nana. Leathesia nana was collected in Weihai of Shandong Province, China in May 2002. The specimen was identified by Dr. Kui-Shuang Shao (Institute of Oceanology, Chinese Academy of Sciences, China). A voucher specimen (No.200216) is deposited at the Herbarium of Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. Brown alga Leathesia nana (8.25 kg, dry weight) was extracted with 95% EtOH at room temperature for 3×72 h. After the solvent was removed under reduced pressure at <40°C, a dark residue (710 g) was obtained. The residue was suspended in water and then partitioned with EtOAc. The EtOAc soluble fraction (125g) was chromatographed over silica gel eluting with a gradual increasing Me_2CO (0%—100%) in petroleum ether. The fraction eluted by 33% Me_2CO in petroleum ether was decolored by column chromatography over Bio-Beads SX3 using CHCl_3-EtOAc (1∶2) and re-chromatographed over Sephadex LH-20 using petroleum ether-CHCl_3-MeOH (5∶5∶1) to afford three subfractions. Separation of the subfractions yielded compounds 1—7 by reverse phase preparative HPLC using MeOH-H_2O-AcOH (75∶25∶0.1) as mobile phase. Column chromatography was performed with silica gel (160-200 mesh, Qingdao Marine Chemical Inc. China) and Sephadex LH-20 (Pharmacia Biotech AB, Uppsala Sweden). HPLC separation was performed on a chromatograph consisting of Waters 600 C

关 键 词：褐藻 小粘膜藻 化学成分 结构鉴定 

分 类 号：R931.77[医药卫生—生药学]