神经生长液对兔周围神经损伤的修复作用研究  

The regenerative effect of nerve growth decoction on the crushed peripheral nerve of rabbits

作　　者：张俊芳[1] 周鸣鸣[1] 刘梅[1] 丁斐[1] 

机构地区：[1]南通医学院江苏省神经再生重点实验室,江苏南通226001

出　　处：《中国临床解剖学杂志》2005年第1期83-86,共4页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(30070255)江苏省高校产业化基金(JH02-116)项目

摘　　要：目的:研究神经生长液(nerve growth decoction,NGD)促进兔周围神经损伤的修复作用。方法:大耳白兔40只,雌雄各半,行腓总神经夹伤术。实验分为五组:NGD低、中、高剂量组,弥可保组(阳性对照),空白对照组。口服给药四周后行电生理、组织学检测和超微结构观察。结果:NGD高、中剂量组和弥可保组在腓总神经传导速度恢复率,脊髓前角运动神经元的存活率及再生髓鞘计数均明显好于空白对照组(P<0.01)。超微结构观察给药组有髓神经纤维的髓鞘形态、厚度、成熟度均优于对照组,变性纤维的数目少于对照组。结论:神经生长液能促进损伤周围神经的再生和功能的恢复。Objective: To explore the regenerative effect of nerve growth decoction (NGD) on the crushed peripheral nerve of rabbits. Methods: Forty rabbits suffered peroneal nerve crush were used in the experiment. The animals were randomly divided into five groups: three NGD groups (low, moderate and high dose), methycobal group (positive control) and control group. Electrophysiology, histochemistry and ultra-structure were studied after treating for four weeks. Results: Compared with the control group, NGD groups (middle and high dose) and methycobal group showed higher recovery ratio of the conduct velocity of the peripheral nerve, higher survival ratio of anterior horn motor neuron and more regenerated myelinated nerve fibers(P<0.01).The number of regeneration nerve fibers, thickness and degree of maturity of .regeneration myelinated nerve sheath in the NGD and methycobal groups were more than those in the control group, but the number of degeneration nerve fibers following nerve injury was less than that in the control group under electron microscope. Conclusions: NGD promotes the regeneration of peripheral nerve and the recovery of function.

关 键 词：神经生长液/药理学 神经再生/中药疗法 周围神经损伤 兔 弥可保 

分 类 号：R285.5[医药卫生—中药学] R743.3[医药卫生—中医学]