脂肽的分离纯化与结构研究  

Isolation and Identification of a Lipopeptide

作　　者：吕应年[1] 杨世忠[1] 牟伯中[1] 

机构地区：[1]华东理工大学化学系,上海200237

出　　处：《微生物学通报》2005年第1期67-73,共7页Microbiology China

基　　金：国家自然科学基金资助项目(No.59974014);高等学校博士学科点专项科研基金资助课题(No.20030251002);上海市教委资助项目~~

摘　　要：采用酸沉淀分离、有机溶剂提取、分级沉淀、脱色吸附及制备薄板的方法,从枯草芽孢杆菌HSO121的培养液中分离得到一种脂肽类化合物。茚三酮试验、红外光谱分析表明该脂肽具有环状结构,电喷雾离子化质谱结果显示,该脂肽是分子量为1022D和1036D的两个同系物,氨基酸分析及串联质谱分析表明,脂肽的肽链结构为Leu-Leu-AspValLeu-Leu-Glu,说明它是Surfactin的结构类似物。A lipopeptide compound was isolated from the culture of Bacillus subtilis HSO121 by methods of acidic precipitation, solvent extract, fractional precipitation, adsorption and prepared thin-layer chromatography; and its molecular structure was determined by by ninhydrin assay and IR methods following the Amino analysis, MS-MS and ESI-MS. It shows that the isolated lipopeptide consists of two homologues with molecular mass 1,022D and 1,036D and bearing a cyclic structure with the amino acid sequence Leu-Leu-Asp-Val-Leu-Leu-Glu in the peptide chain, which indicates that the isolated lipopeptide falls into the analogs of surfactin.

关 键 词：枯草芽孢杆菌 脂肽 分离纯化 环状结构 

分 类 号：TQ28[化学工程—有机化工]