药物代谢酶CYP3A在中国肾移植人群的遗传多态性  

Genetic polymorphism of CYP3A in Chinese renal transplant population

作　　者：储小曼[1] 闵佩清[1] 张静[1] 孔诚[1] 蔡卫民[1] 

机构地区：[1]南京军区南京总医院临床药理科,江苏南京210002

出　　处：《医学研究生学报》2005年第2期107-111,共5页Journal of Medical Postgraduates

摘　　要：目的:建立药物代谢酶CYP3A4、CYP3A5的多个等位基因分型方法,为进一步从基因水平研究CYP3A与机 体对药物反应的个体差异本质及其与药物代谢的相关性提供分子生物学实验方法;探讨药物代谢酶CYP3A在中 国肾移植人群的遗传多态性。　方法:用聚合酶链反应 限制性片段长度多态性(PCR RFLP)法及特异性等位基因 扩增(PCR ASA)法分别建立了CYP3A的CYP3A4亚型三个新突变点(CYP3A4 4、 5、 6)及CYP3A5亚型的 CYP3A5 3基因分型方法,并对中国肾移植人群进行基因分型。　结果:CYP3A4 4、 5、 6、CYP3A5 3在中 国肾移植人群的基因频率分别为0.75%、0.76%、0.75%和27.83%;个体突变率分别为2/133、3/197、3/200和 55/115。　结论:研究结果提示,中国肾移植人群中CYP3A4 4、 5、 6、CYP3A5 3的存在可能使其表达的药 物代谢酶活性改变,从而影响药物在体内的代谢过程。Objective: To establish the methods for determining CYP3A genotype in order to further research the influence of CYP3A genetic polymorphism on drug metabolism and reaction; to report the genotype distribution of CYP3A4*4、*5、*6 and CYP3A5*3 in Chinese renal transplant population. Method: CYP3A genotype was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and PCR amplification of specific alleles(PCR-ASA).We analyzed the samples from Chinese renal transplant recipients. Results: The allele CYP3A4*4 was found in 2 of 133 subjects .The allele CYP3A4*5 was found in 3 of 197 subjects. The allele CYP3A4*6 was found in 3 of 200 subjects. And the allele CYP3A5*3 was found in 55 of 115 subjects.And the gene frequencys of CYP3A4*4、*5、*6 and CYP3A5*3 were 0.75%,0.76%,0.75% and 27.83%,respectively. Conclusion: This pilot study suggests that the effect of CYP3A genetic polymorphism may decrease the CYP3A activity that affected the drug metabolism and reaction.

关 键 词：CYP3A 基因多态性 药物代谢 特异性等位基因扩增法 聚合酶链反应-限制性片段长度多态性 

分 类 号：R699.2[医药卫生—泌尿科学]