苯丙氨酸缓解高血压大鼠血管重塑  

L-phenylalanine ameliorates the vascular remodeling in hypertensive rats

作　　者：车在前[1] 顾天华[1] 吴永杰[1] 赵光胜[1] 

机构地区：[1]上海第二医科大学附属瑞金医院上海市高血压研究所,上海200025

出　　处：《中国病理生理杂志》2005年第2期327-330,共4页Chinese Journal of Pathophysiology

基　　金：上海第二医科大学自然科学研究基金项目(No .2 0 0 1校 0 5 )

摘　　要：目的 :应用激光共聚焦显微镜观察高血压时血管重塑 ,以及苯丙氨酸治疗后对血管重塑的影响。方法 :以Wistar-Kyoto大鼠 (WKY)和自发性高血压大鼠 (SHR)为研究对象 ,采用固定压力灌注和荧光染色激光共聚焦显微镜观察分析肠系膜小动脉的管腔、厚度和血管平滑肌的排列方向。结果 :SHR较WKY管腔缩小 ,管壁增厚 ,同时血管平滑肌排列方向紊乱。应用苯丙氨酸治疗后 ,管腔扩大 ,管壁变薄 ,血管平滑肌排列方向改善。结论 :高血压大鼠肠系膜小动脉存在血管重塑现象 ,苯丙氨酸可以改善血管重塑。AIM: To investigate the effect of L-phenyla lanine on vascular remodeling in hypertensive rats. METHODS: Vascular remodeling was measured by laser scanning conf ocal microscopy (LSCM) in mesenteric resistance arteries isolated from spontaneo usly hypertensive rat (SHR) and normotensive Wistar-Kyoto (WKY) rat. The effect of L-phenylalanine on the hypertensive vascular remodeling was observed. The thi rd most distal first-order branches of mesenteric resistance arteries from SHR a nd WKY were studied. The arteries were fixed under pressure. The segments were s tained with the nuclear dye propidium iodide. The diameter, wall thickness and o rientation angle of smooth muscle cells were measured with LSCM. RESULTS: Compared with WKY, SHR arteries showed: (1) smaller lum en, (2) increased wall thickness, (3) disorganized orientation angle of smooth m uscle cells. L-phenylalanine treatment induced specific changes in the lumen, wa ll thickness and the orientation angle of smooth muscle cells. CONCLUSIONS: Hypertension induces vascular remodeling of the bra nches of mesenteric arteries from SHR. L-phenylalanine inhibits the vascular rem odeling process of hypertension.

关 键 词：显微镜检查 共焦 苯丙氨酸 高血压 血管重塑 

分 类 号：R331.3[医药卫生—人体生理学]