变形梯度胶电泳分析喂养方式对早产新生儿肠道菌群的影响  

Study of effects on intestinal microbiota between breast-feeding and formula-feeding premature neonates by PCR-denaturing gradient gel electrophoresis

作　　者：兰和魁[1] 符薇[1] 陈丽珊[1] 李秀云 王霞[3] 严奇[1] 任大明[1] 

机构地区：[1]复旦大学遗传所,上海200433 [2]广东省一七七医院,广东广州510315 [3]南方医科大学附属珠江医院,广东广州510282

出　　处：《中国微生态学杂志》2005年第1期5-7,共3页Chinese Journal of Microecology

摘　　要：目的采用变性梯度胶电泳(DGGE)方法研究喂养方式对早产新生儿肠道菌群的影响。方法收集同期6对新生儿1～21d粪便,直接提取细菌总DNA,扩增16SrDNAV6～V8区后DGGE分离,测序并与EMBL核苷序列数据库进行比较。结果喂养前肠道菌群类似,以梭状芽胞杆菌、链球菌和克雷伯菌为主;开奶后母乳喂养儿以双歧杆菌为主,奶粉喂养儿肠道菌群显示其明显的多态性,有双歧杆菌、梭状芽胞杆菌、链球菌、大肠埃希菌、克雷伯菌、韦荣菌、沙雷菌以及不经培养细菌。结论喂养方式对早产新生儿菌群的形成及演替有明显影响,PCRDGGE在多态性,动力性,菌群的演进变化方面提供了更加准确的数据和补充资料。Objective To study the difference of intestinal microbiota between breast-feeding and formula-feeding premature neonates. Methods Total DNAs were extracted from the feces of two group neonates.Bacterial communities in two group infants were examined by PCR of 16S rDNA V_6～V_8 regions and denaturing gradient gel electrophoresis (DGGE) and by analyzing the sequences of the ribotypes.Results During the first few days of life the profiles of DGGE were simple,but they became more complex as the bacterial diversity increased with time,especially in formula-feeding infants.Species most closely related to the Bifidobacterium dominated the intestinal microbiota in breast-feeding infants.Bacterial diversity were more marked in formola-feeding infants than breast-feedingg.The bacteria identified included members of genera Bifidobacteria,Clostridium,E.coli,Streptococcus,Uncurtured bacteria,Klesiella,Veillonella,Uncultured fecal bacterium,Serratia.Conclusion Feeding mode was a important factor affecting developmental intestinal microbiota in premature neonates.PCR-DGGE and 16S rDNA sequence analysis resulted in a dynamic description of bacterial ciloniztioni n the infant intestinal microbiota and allowed visualization of bacteria that were difficult to cultivate or to detect by other methods.

关 键 词：早产新生儿 喂养方式 肠道菌群 克雷伯菌 梭状芽胞杆菌 影响 多态性 电泳分析 链球菌 总DNA 

分 类 号：R722.1[医药卫生—儿科] R174[医药卫生—临床医学]