日本血吸虫肌球蛋白部分重链基因核酸疫苗的构建与小鼠保护性研究  

Immunogenicity of the plasmid DNA encoding Schistosoma japonicum myosin heavy chain

作　　者：李小红[1] 刘述先[1] 宋光承[1] 徐裕信[1] 曹建平[1] 陈家旭[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所

出　　处：《中国血吸虫病防治杂志》2005年第1期9-12,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家863高技术计划项目(No.10207040197)

摘　　要：目的研究日本血吸虫肌球蛋白部分重链基因核酸疫苗对小鼠的保护效果。方法用致弱童虫免疫血清筛选日本血吸虫成虫cDNA文库,获得的阳性克隆之一与曼氏血吸虫肌球蛋白重链基因同源。PCR法扩增该片断基因,产物克隆入真核表达载体pcDNA3中,构建成肌球蛋白部分重链基因核酸疫苗。将50μg肌球蛋白(myosin)核酸疫苗注射C57BL/6小鼠的两侧股四头肌,免疫3次,间隔2周,末次免疫后2周经腹部皮肤攻击感染尾蚴30条/鼠,感染6周后用门静脉灌注法收集成虫,计算核酸疫苗免疫组减虫率与空质粒对照组减虫率。结果核酸疫苗免疫组获得了33.02%的减虫率,空质粒对照组获得了24.50%的减虫率,经t检验,两组差异无显著性。结论肌球蛋白部分重链基因核酸疫苗在小鼠中未能诱导出保护力。Objective To study the efficacy of part myosin heavy chain DNA vaccine of Schistosoma japonicum in mice. Methods The cDNA fragment encoding part-length heavy chain of myosin in S.japonicum, obtained by immuno-screen with the ultraviolet-attenuated schistosomula immunized sera, was cloned into the vector pcDNA3 to construct a DNA vaccine. Fifty microgram of this DNA vaccine was injected into C_~57 BL/6 mouse quadriceps muscle of both sides. The procedure was three immunizations with 2 weeks interval, and a challenge with 30 cercariae/mouse two weeks post-immunization. Adult worms were harvested 6 weeks after the challenge through portal vein perfusion. Worm reduction rates were calculated. Results The mice immunized with the nucleic acid vaccine showed 33.02% reduction rate of worm, and immunized with only plasmid DNA was 24.50% reduction rate of worm compared with the blank vector and there was no significance in statistics between two groups. Conclusion The nucleic acid vaccine constructed in this experiment failed to induce significant protection against the challenge of S.japonicum infection in mice.[

关 键 词：日本血吸虫 肌球蛋白 核酸疫苗 

分 类 号：R383.24[医药卫生—医学寄生虫学]