浙江省6种短沟蜷足肌全蛋白SDS-PAGE图谱的比较研究  

SDS-PAGE for foot proteins of Semisulcospira in Zhejiang Province

作　　者：钱宝珍[1] 

机构地区：[1]浙江省医学科学院生物工程研究所,杭州310013

出　　处：《中国血吸虫病防治杂志》2005年第1期49-51,共3页Chinese Journal of Schistosomiasis Control

基　　金：国家科技部中国-泰国长期合作项目"肺吸虫病的研究"(ID:17-603J)

摘　　要：目的探讨短沟蜷属螺类生化特性和种间差异,以及对传播卫氏并殖吸虫病的意义。方法采用SDS-聚丙烯酰胺凝胶电泳(PAGE),对浙江省6种短沟蜷(Semisulcospira)螺类的足肌全蛋白区带进行比较研究。结果各螺种出现率在50%以上的多肽区带为17～20条,分子量在27～162kDa之间。其中仅有2条强多肽区带完全一致,分子量为93kDa和52kDa。各种间特征性区带主要表现在其染色特性上,全蛋白种间相似系数(Sm)在0.74～0.85之间。结论结合短沟蜷属螺类形态学特点,两条多肽主带,可作为该属的生化分类指标,对传播人体并殖吸虫病可能具有意义。Objective To understand the biochemical characters of the intra-species of Semisulcospira and importance of paragonimiasis transmission. Methods The foot proteins of six species of Semisulcospira in Zhejiang Province were studied and compared by SDS-PAGE. Results 17-20 protein bands were found with molecular weight 27-162 kDa. Two strong bands,93 kDa and 52 kDa, were the same among the six species of Semisulcospira. The similitude coefficient was 0.74-0.85. Conclusion Two strong bands,93 kDa and 52 kDa, would be the protein markers of Semisulcospira, which may be related to the transmission of paragonimiasis.[

关 键 词：短沟蜷属 聚丙烯酰胺凝胶电泳 并殖吸虫 浙江 

分 类 号：R383.23[医药卫生—医学寄生虫学]