毛细管电泳研究HIV抑制剂、Tat蛋白与RNA的相互作用  

Study on the Interaction of Human Immunodeficency Virus Inhibitor, Trans-activitor of Transcription Protein and Ribonucleic Acid System by Capillary Electrophoresis

作　　者：齐小花[1] 丁里[1] 张新祥[1] 常文保[1] 林伟[2] 杨铭[2] 

机构地区：[1]北京大学化学与分子工程学院,北京100871 [2]北京大学医学部药学院,北京100083

出　　处：《分析化学》2005年第2期214-216,共3页Chinese Journal of Analytical Chemistry

摘　　要：利用毛细管区带电泳法对新型HIV抑制剂(β 咔啉类药物)、HIVTat蛋白与HIVTARRNA竞争作 用中的反应物和产物进行分离,研究了这种新型HIV抑制剂与RNA的相互作用,证明了其结合比为1∶1,并 进一步测定了该反应的结合常数为2.76×104L/mol,与Tat蛋白和RNA的结合常数相近,说明这种抑制剂可 与蛋白竞争性地结合RNA,从而有效抑制病毒复制。此结果与生物学方法的结论一致。The reagents and products of the competitive reaction of a new type of human immunodeficency virus (HIV) inhibitor (β-carboline drug), HIV trans-activitor of transcription (Tat) protein and HIV trans-activitor response region (TAR RNA) by capillary zone electrophoresis were separated, and the interaction of HIV inhibitor and RNA was studied. The combining ratio of 1∶ 1 was determined. In addition, the binding constant of the interaction was 2.76 ×10 4 L/mol, and it was the same as that of Tat protein and RNA. The HIV inhibitor interacted with RNA with competitive reaction of Tat protein, and it restrained virus reproduction effectively. The result was consistent with that of biology method.

关 键 词：HIV RNA TAT蛋白 抑制剂 病毒复制 毛细管区带电泳法 药物 结合常数 相互作用 毛细管电泳 

分 类 号：R346[医药卫生—基础医学]