离子对缔合物萃取分光光度法测定维生素K_3  

Extraction-Spectrophotometric Determination of Vitamin K_3 by Ion-pair Complexes

作　　者：陈彩萍[1] 杜黎明 段亚丽[1] 张艳 

机构地区：[1]山西师范大学化学与材料科学学院,临汾041004 [2]山西师范大学分析测试中心,临汾041004

出　　处：《分析化学》2005年第2期237-240,共4页Chinese Journal of Analytical Chemistry

基　　金：山西省自然科学基金资助课题(No.20041030)

摘　　要：研究了维生素K3(vitaminK3,VK3)与碱性染料丁基罗丹明B(butylrhodamineB,BRB)、乙基紫 (ethylviolet,EV)和亚甲基蓝(methyleneblue,MB)形成离子对缔合物的反应,确定了反应的最佳条件。建立 了测定VK3含量的简单、快速、选择性好和灵敏度高的分析方法。VK3与BRB、EV及MB反应生成可被有机 溶剂萃取的离子对缔合物,离子对缔合物分别在568、613和658nm处有最大吸收,其表观摩尔系数分别为 2.92×104、6.96×104和4.85×104L·mol-1·cm-1;离子对缔合物的组成均为1∶1,稳定常数分别是9.5× 105、1.9×106和7.8×105;药物浓度分别在0.0083～13、0.029～6.6和0.015～9.6mg/L范围内符合比尔定 律;回收率为96%～103%。该法可成功地用于药物制剂中VK3含量的测定。A simple, selective and sensitive method was developed for the extractive spectrophotometric determination of vitamin K-3 (VK3). The method was based on the formation of ion-pair complexes, VK3 with the various basic dye: butyl rhodamine B (BRB), ethyl violet (EV) and methylene blue (MB). The ion-pair complexes of BRB-VK(3)and EV-VK3 can be extracted into benzene and has absorption maximum respectively at 568 run and 613 nm respectively, The ion-pair complex of VK3-MB can be extracted into 1,2-dichloroethane, and has absorption maximum at 658 nm. The calibration graphs generated were linear over the, range of 0.083 similar to 13. mg/L, 0.029 similar to 6. 6 mg/L, 0.015 similar to 9.6 mg/L drug in solution respectively with. epsilon(VK3-BRB) = 2.9 x 10(4) L center dot mol(-1) center dot cm(-1), epsilon(VK3-EV) =6.96 X 10(4) L center dot mol(-1) center dot cm(-1), epsilon(VK3-MB) = 2.99 X 10(4) L center dot mol(-1) center dot cm(-1). The recovery of method is 97% similar to 103%. The proposed method has been successfully applied to the determination of VK3 in pharmaceutical formulations.

关 键 词：离子对 维生素K3 MB 含量 VK 反应 药物浓度 缔合物 稳定常数 萃取 

分 类 号：TQ465[化学工程—制药化工]