天花粉蛋白在不同小鼠品系中区分性下调树突状细胞IL-12分泌和CD80表达  

Trichosantin differentially down regulates dendritic cells for their secretion of IL-12 and the expression of costimulation molecule CD80 in the high and low susceptible mouse strains

作　　者：王保龙[1] 周芸[1] 江阳[1] 辛利军[1] 周洪[1] 周光炎[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,上海200025

出　　处：《中国免疫学杂志》2005年第2期89-92,共4页Chinese Journal of Immunology

基　　金：国家自然基金项目 (3 0 2 71616);国家 863项目 (2 0 0 1CB5 10 0 0 3 )资助

摘　　要：目的 :阐明天花粉蛋白 (Tk)诱导免疫抑制和基因调控的机制。方法 :同时在高易感品系 (HS)和低易感品系(LS)小鼠中 ,应用淋巴细胞体外增殖试验测定Tk对DC细胞递呈抗原和激活T细胞的影响、ELISA方法测定TK对DC细胞分泌IL-12p4 0的影响、流式细胞术测定TK对DC细胞表面MHCⅡ类分子及共刺激分子 (CD80、CD86、CD4 0 )和CD11c表达的影响。结果 :(1)Tk抑制高易感品系C5 7BL 6 (H 2 b)DC细胞的抗原递呈和激活T细胞的能力 ,对低易感品系C3H He(H-2 k)影响甚小 ;(2 )Tk抑制C5 7BL 6DC细胞成熟过程中IL-12p4 0的分泌 ,对C3H He的抑制减弱 ;(3)Tk选择性下调C5 7BL 6DC细胞CD80的表达 ,对C3H He无明显影响 ;(4)DC细胞表面CD4 0、CD86及CD11C分子的表达在两种小鼠品系中均不受Tk的影响。结论 :Tk选择性地在HS小鼠品系中下调DC细胞IL-12和CD80的表达 。Objective:To elucidate the mechanisms underlying the Trichosanthin (Tk)-induced immune suppression and its genetic control.Methods:Lymphoproliferation in vitro was adopted to measure the effects of Tk on dendritic cells for their ability of presenting antigen and activating T cells in high susceptible (HS) and low susceptible (LS) mouse strains. Bone marrow-derived immature dendritic cells (iBDCs) were treated with LPS (1 μg/ml) and recombinant mouse IFN-γ (20 ng/ml) for maturation. The concentration of IL-12 p40 in culture supernatants were detected by ELISA for the mature BDCs cultured with or without Tk. The expression of H-2 classⅡ, CD80, CD86, CD40 and CD11c molecules on BDCs were also determined by FACS.Results:For HS mouse strain (C57BL/6),the ability of BDCs for presenting and activating T cells was suppressed significantly in compared with a little suppression found in LS (C3H/He). The secretion of IL-12p40 was also decreased in C57B1/6 but not in C3H/He when the BDCs were cultured with Tk in LPS-containing medium. The expression of costimulatory molecule CD80 was down regulated for C57BL/6 and no change for C3H/He when Tk was added in the course of iBDC maturation. The expressions of other costimulation molecules we detected seemed not to be affected by Tk treatment both in HS and LS.Conclusion:Tk's ability of inducing suppression of T cell responses might depend on certain immunological condition of antigen-presenting cells, including their secretion of key cytokines and the expression of some costimulation molecules. All of them might be under the genetic control of MHC- related genes since there are quite differential patterns in responding to Tk stimulation for HS and LS mouse strains.

关 键 词：天花粉蛋白 DC细胞 IL-12 P40 共刺激分子 免疫应答的遗传控制 

分 类 号：R392.12[医药卫生—免疫学]