脂肪组织中胰岛素受体底物1蛋白的表达及酪氨酸磷酸化在多囊卵巢综合征发病中的作用  

Expression of insulin receptor substrate 1 and phosphorylation of tyrosine in adipose tissue of polycystic ovary syndrome

作　　者：王竹晨[1] 顾熊飞[2] 杨冬梓[1] 邝健全[1] 

机构地区：[1]中山大学附属第二医院妇产科 [2]中山大学生物化学教研室,广州510120

出　　处：《中华妇产科杂志》2005年第2期120-123,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：广东省卫生厅科研基金资助项目(2001189)

摘　　要：目的探讨多囊卵巢综合征(PCOS)患者脂肪组织胰岛素受体底物1(IRS1)的蛋白表达及酪氨酸磷酸化在PCOS发病中的作用。方法采用免疫沉淀法、Western印迹法和增强化学发光蛋白免疫印迹法及图像分析半定量检测24例PCOS患者[PCOS组,根据体重指数分为肥胖(体重指数≥24kg/m2)和非肥胖(体重指数<24kg/m2)者各12例]及同期因卵巢囊肿或输卵管阻塞行开腹手术的非PCOS患者24例(对照组,根据体重指数分为肥胖和非肥胖者各12例)的脂肪组织中IRS1的蛋白表达及酪氨酸磷酸化程度。结果PCOS组肥胖者IRS1的蛋白表达为(82±15)%,PCOS组非肥胖者为(79±18)%;对照组肥胖者为(75±19)%,对照组非肥胖者为(70±19)%,各组间比较,差异均无统计学意义(P>005)。脂肪组织中IRS1的酪氨酸磷酸化程度在PCOS组肥胖者为(52±23)%,对照组非肥胖者为(88±12)%,两者比较,差异有统计学意义(P<001);对照组肥胖者为(45±22)%,PCOS组非肥胖者为(70±25)%,与对照组非肥胖者比较,明显降低,差异均有统计学意义(P<001,P<005);PCOS组肥胖者和对照组肥胖者间以及PCOS组肥胖者和PCOS组非肥胖者间比较,差异均无统计学意义(P>005)。结论PCOS组脂肪组织IRS1的蛋白表达及酪氨酸磷酸化程度明显减弱,可能参与胰岛素受体后信号传导抑制,并与PCOS胰岛素抵抗的发生有?ObjectiveTo study expression of insulin receptor substrate 1 (IRS-1) and phosphorylation of tyrosine in adipose tissue of polycystic ovary syndrome (PCOS) to approach the mechanism of insulin resistance (IR) in PCOS at tissue and cellular level. MethodsIRS-1 expression and tyrosine phosphorylation of adipose tissue were studied with immunoprecipitation, Western-blot and ECL immunoblotting. ResultsThe differences of IRS-1 expression in adipose tissue among the obese PCOS group (82±15)%, the non-obese PCOS group (79±18)%, the obese control group (75±19)% and the non obese control group (70±19)% were not significant (P>0.05). IRS-1 tyrosine phosphorylation in adipose tissue in the obese PCOS group [(52±23)%, P<0.001], the obese control group [(45±22)%, P<0.01] and non-obese PCOS group [(70±25)%, P<0.05] were markedly lower than that in the non-obese control group (88±12)%. The obese control group had more reduction than non-obese control group (P<0.05). The differences between the obese PCOS group and the obese control group or the two PCOS groups were not significant (P>0.05). ConclusionIRS-1 tyrosine phosphorylation in adipose tissue in the two PCOS groups decreased markedly than in the non-obese control group. It might be involved in the disorder of insulin-signaling transduction downstream of insulin receptor and the occurrence of IR.

关 键 词：肥胖者 PCOS 对照组 酪氨酸磷酸化 IRS-1 脂肪组织 蛋白表达 蛋白免疫印迹法 信号传导 免疫沉淀法 

分 类 号：R711.7[医药卫生—妇产科学]