丹皮多糖2b对体外非酶糖化反应及终产物生成的抑制作用  

Inhibitory effect of PSM_(2b) from Paeonia suffruticosa on NEG and AGEs in vitro

作　　者：刘长安[1] 赵帜平[1] 沈业寿[1] 戴玲[1] 

机构地区：[1]安徽大学生命科学学院糖复合物与糖工程实验室,安徽合肥230039

出　　处：《生物学杂志》2005年第1期20-21,共2页Journal of Biology

基　　金：安徽省"十五"重大科技专项基金(01803011);国家自然科学基金(302400661);安徽省自然科学基金(03043301)。

摘　　要：建立体外非酶糖化系统,即加入200mmolL-1Glc,40gL-1牛血清白蛋白,另加0.01%NaN3抗菌,再加入PSM2b使其终浓度分别为1,0.5,0.1,0.01mgmL 1。37℃保温,温孵2周后测定对非酶糖化反应抑制率(IR1),8周后测定对AGEs产物生成的抑制率(IR2)。研究丹皮多糖2b(PSM2b)对体外非酶糖化反应和终产物AGEs的生成均有较高抑制作用。其IR1和IR2最高分别为23.51%和56.51%。PSM2b对非酶糖化引起的糖尿病微血管病变有改善作用。To investigate the inhibitory effects of PSM_(2b) from Paeonia suffruticosa on nonenzymatic glycosylation of protein in vitro.NEG were prepared in vitro by incubation of bovine albumin(40g L^(-1)),glucose(200 mmol L^(-1))and 0.1% NaN_3 with PSM_(2b),PSM_(2b-A)in different concentration(1,0.5,0.1,0.01 mg mL^(-1)) at 37℃.After 15 days early glycosylation products were measured and after 8 weeks AGEs were measured.The inhibiting rate of PSM_(2b) to NEG reached 27.56%,to AGEs reached (56.51%).PSM_(2b) and PSM_(2b-A) could improve chronic complications of diabetes.

关 键 词：丹皮多糖 非酶糖化反应 非酶糖化终产物 抑制 

分 类 号：Q53[生物学—生物化学] R282.71[医药卫生—中药学]