麻黄药材的质量分析研究  

Quality Analysis of Herba Ephedra

作　　者：林朝展[1] 祝晨陈[1] 杨金燕[1] 陈康[1] 

机构地区：[1]广州中医药大学中药学院,广州510405

出　　处：《中药新药与临床药理》2005年第1期56-58,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家"十五"科技攻关项目:麻黄炮制规范化研究(编号2001BA701A11)。

摘　　要：目的通过对多批次草麻黄药材质量进行分析比较,确定可行的麻黄碱与伪麻黄碱的含量限度,为其质量控制提供科学依据,为麻黄药材质量分析提供新方法。方法采用RP-HPLC法测定麻黄碱、伪麻黄碱的含量,KromasilRP-C_(18)色谱柱(4.6mm×250mm,5μm),流动相:0.3%磷酸-甲醇(10∶90),检测波长:213nm。结果14批草麻黄药材中麻黄碱及伪麻黄碱含量差异较大,其中麻黄碱含量在0.361～1.538%,伪麻黄碱在0.332～2.087%之间。通过多批次样品含量比较,建议草麻黄药材中麻黄碱的含量不低于1.082%,伪麻黄碱含量不低于1.008%。结论经过系统的方法学考察,所建立的测定方法具有操作简便、稳定、专属、可重复的特点,可用于麻黄药材及含麻黄复方制剂的质量控制。Objective To supply evidence for quality control of Herba Ephedra by determining content limit in different batches of medicinal material of Herba Ephedra.Methods A RP-HPLC method was used for the determination of ephedrine and pseudo-ephedrine. The chromatographic conditions were: Kromasil RP-C18(250×4.6 mm,5 μm) column, a mixture of 0.3 %phosphoric acid-methanol (10∶90) as mobile phase and 213nm as detected wavelengths.Results The contents of the above two alkaloids showed great difference among the 14 patches of tested samples. The content of ephedrine ranged 0.361 %～1.538 %and that of pseudo-ephedrine 0.332～2.087 %. The suggested content limitation of ephedrine should be 1.082 %, and that of psedoephedrine be 1.008 %in the crude Herba Ephedra.Conclusion The established method has been proven to be simple, stable and repeatable, and can be applied for quality control of the crude crude Herba Ephedra and compound prescriptions containing crude Herba Ephedra.

关 键 词：麻黄 麻黄碱 伪麻黄碱 含量测定 RP-HPLC法 

分 类 号：R284.1[医药卫生—中药学]