Generation of mutants with developmental defects in zebrafish by ENU mutagenesis  

作　　者：JINPeng TIANTian SUNZhihui MENGAnming 

机构地区：[1]LaboratoryofDevelopmentalBiology.DepartmentofBiologicalSciencesandTechnology.TsinghuaUniversity.Beijing100084.China

出　　处：《Chinese Science Bulletin》2004年第20期2154-2158,共5页

摘　　要：As a good model for studying early develop-ment of vertebrates, zebrafish (Danio rerio) is attracting more and more attention. Following ENU mutagenesis, 320 F2 families were established. Mutants, which showed defects in epiboly, axis, somite, head, and cardiac and blood systems, were identified by observing morphological changes in F3 embryos. So far, 35 mutant lines have been established, the majority of which showed anomalies in axis and somite for-mation. These mutant lines provide useful genetic resources for cloning of the mutant genes and for studying mechanisms of early development of vertebrate embryos.As a good model for studying early develop-ment of vertebrates, zebrafish (Danio rerio) is attracting more and more attention. Following ENU mutagenesis, 320 F2 families were established. Mutants, which showed defects in epiboly, axis, somite, head, and cardiac and blood systems, were identified by observing morphological changes in F3 embryos. So far, 35 mutant lines have been established, the majority of which showed anomalies in axis and somite for-mation. These mutant lines provide useful genetic resources for cloning of the mutant genes and for studying mechanisms of early development of vertebrate embryos.

关 键 词：突变异种 斑马鱼 ENU诱变 脊椎动物 晶胚 

分 类 号：Q959.483[生物学—动物学] Q953.3