Cloning and analysis of expression profile of 13 WRKY genes in rice  

作　　者：QIUYuping JINGShaojuan FUJian LILu YUDiqiu 

机构地区：[1]KunmingSection,XishuangbannaTropicalBotanicalGarden,TheChineseAcademyofSciences,Kunming650223,China [2]GraduateSchooloftheChineseAcademyofSciences,Beijing100039,China

出　　处：《Chinese Science Bulletin》2004年第20期2159-2168,共10页

摘　　要：The transcriptional factor WRKY proteins contain the highly conserved amino acid sequence WRKYGQK as well as the novel zinc-finger-like motifs Cys2His2 or Cys2HisCys. A search of the rice genome identi-fied 97 genes encoding WRKY proteins. Of these 97 WRKY homologs found in rice, 13 cDNAs encoding WRKY proteins were consequently isolated from a rice cDNA library con-structed from 4℃-treated shoots by probing for the con-served WRKY domain. Northern blotting analysis revealed that 10 of 13 OsWRKY genes were differentially regulated in plants that were treated by the four following abiotic stress factors: NaCl,PEG,cold (4℃) and heat (42℃). The resulting expression profiles exhibited great differences in both the manner and timing of their response to the four different abiotic treatments. The difference of gene expression profiles suggested the different physiological functions among the WRKY genes.The transcriptional factor WRKY proteins contain the highly conserved amino acid sequence WRKYGQK as well as the novel zinc-finger-like motifs Cys2His2 or Cys2HisCys. A search of the rice genome identi-fied 97 genes encoding WRKY proteins. Of these 97 WRKY homologs found in rice, 13 cDNAs encoding WRKY proteins were consequently isolated from a rice cDNA library con-structed from 4℃-treated shoots by probing for the con-served WRKY domain. Northern blotting analysis revealed that 10 of 13 OsWRKY genes were differentially regulated in plants that were treated by the four following abiotic stress factors: NaCl,PEG,cold (4℃) and heat (42℃). The resulting expression profiles exhibited great differences in both the manner and timing of their response to the four different abiotic treatments. The difference of gene expression profiles suggested the different physiological functions among the WRKY genes.

关 键 词：无性繁殖 稻 基因表达 WRKY基因 转录因子 非生物应激因子 转基因作物 

分 类 号：S511[农业科学—作物学] S188