缺血预适应远程保护作用在缺血脊髓的实验研究  

Experimental study on the remote protective effects of ischemic preconditioning on spinal cord ischemia/reperfusion in rabbits

作　　者：徐浩[1] 管华清[1] 张亚峰[1] 杨惠林[1] 杨淮海 

机构地区：[1]苏州大学附属第一人民医院骨科,江苏苏州215006 [2]盐城市第一人民医院骨科

出　　处：《中国急救医学》2005年第2期107-109,共3页Chinese Journal of Critical Care Medicine

摘　　要：目的探讨双后肢缺血预适应(IPC)对兔脊髓缺血/再灌注损伤远程保护作用。方法采用肾下腹主动脉阻断法,建立脊髓缺血/再灌注模型。IPC组先用止血带结扎兔双后肢根部5min,松开5min,重复三次,15min后再阻断腹主动脉35min后开放灌注,缺血组阻断腹主动脉35min后开放灌注。术后进行神经功能评分,观察脊髓标本的病理学改变及细胞凋亡情况。测定脊髓组织中IL-8的水平。结果神经功能评分IPC组在各时间点明显高于缺血组,组织病理学变化各时间点明显好于缺血组,凋亡细胞数及IL-8水平明显低于同时间点缺血组。结论双后肢IPC通过调动机体内源性抗损伤机制对缺血脊髓发挥远程保护作用。Objective To study the remote protection of double rear legs ischemic preconditioning on spinal cord injury following ischemia and reperfusion. Methods The spinal cord ischemia/reperfusion model was made by clamping the infrarenal aorta in rabbits. The IPC group underwent three cycles of 5 minutes of hemostatic strip ligating double rear legs ,5 minutes loosing and 35 minutes of ischemia by clamping the infrarenal aorta. In the ischemia group, the abdominal aorta was occlused for 35 minutes and then reperfused. Neurological outcome was scored after reperfusion. Spinal cord histopathology and apoptosis were examined.The level of IL-8 was measured. Results The neurological function scores at all the time points were significantly higher than those in ischemia group. Histopathologic results were obviously better in IPC group than in ischemic group. The numbers of apoptosis neurons and IL-8 levels significantly decreased at the same time points in contrast to those in ischemic group. Conclusions Double rear legs IPC can protect spinal cord function from ischemia injury by an endogenous cellular protective mechanism. [

关 键 词：脊髓损伤 缺血/再灌注 缺血预适应 凋亡 白细胞介素-8 

分 类 号：R651.2[医药卫生—外科学]