缓激肽β_2受体基因启动子区SSCP带型与新疆哈萨克族人原发性高血压的相关性分析  

The study for association of Genetic variants in the promoter region of the β_2 bradykinin receptor gene and essential hypertension in Kazak of Xinjiang

作　　者：汪迎春[1] 李南方[1] 周玲[1] 李涛[1] 周克明[1] 任荣[1] 

机构地区：[1]新疆维吾尔自治区高血压研究所

出　　处：《新疆医科大学学报》2005年第1期12-14,共3页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(30260038;30240045)

摘　　要：目的:探讨缓激肽β2 受体基因启动子区 91 位至 7 位基因变异与新疆哈萨克族人原发性高血压的关系。方法:运用聚合酶链反应-单链构象多态性分析(PCR SSCP)方法,对 118 例收缩压≥150 mmHg和/或舒张压≥95 mmHg的哈萨克族原发性高血压病人和 65 例年龄、性别、族别、BMI相匹配的血压<135/85 mmHg的正常血压者,分别进行缓激肽β2 受体基因启动子区 91位至 7位基因多态性检测,观察不同SSCP带型在高血压病人组和正常血压对照组中的分布频率。结果:新疆哈萨克族人缓激肽β2 受体基因启动子区存在 A、B、C 3 种带型,A、B、C带型在原发性高血压病人组和正常血压对照组的分布频率分别为 0.28、0.37; 0.22、0.23; 0.50、0.40。3种带型的分布频率在两组间差异无统计学意义(χ2= 2.002, P=0.367)。结论:新疆哈萨克族人缓激肽β2 受体基因启动子区 91位至 7位存在有3种SSCP带型,提示该区域可能存在有基因变异,但该变异可能与哈萨克族人原发性高血压不相关。Objective: To investigate the association between the genetic variants in the promoter region of bradykinin β-2 receptor gene and the essential hypertension in Kazak of Xinjiang. Methods: This study selected 118 hypertensive patients(SBP≥150 mmHg and/or DBP≥95 mmHg)and 65 normotenive controls(BP<135/85 mmHg)matched with sex 、age、BMI from Kazak of Xinjiang. The measurement of the genetic variants was performed by PCR-SSCP in hypertensive patients and normotensive controls respectively. Results: The A、B、C SSCP band patterns were detected in the experiment. The distributed frequencies of the A、B、C SSCP band patterns were 0.28、0.37; 0.22、0.23; 0.50、0.40 respectively in hypertensive group and normotensive group. There was no significant difference between the two groups (χ+2= 2.002,P= 0.367). Conclusion: In Kazak of Xinjiang, there are three different SSCP band patterns in the promoter -91 to -7 region of bradykinin β-2 receptor gene. It suggest thats there exist variants in the promoter region of bradykinin β-2 receptor gene, but the variants may not be associated with essential hypertension.

关 键 词：高血压 缓激肽β2受体基因 哈萨克族 

分 类 号：R544.1[医药卫生—心血管疾病]