氟化钠和酒精对大鼠肝间质的联合毒性作用——肝贮脂细胞和胶原纤维的形态计量学研究  

Combining Toxicity of Sodium Fluoride and Ethanol on the Mesenchyma of Rat Liver——A Morphometric Study of Ito Cells and Collagen

作　　者：何劲松[1] 张乃鑫[2] 章明放[1] 王晓燕[1] 谭郁彬[2] 

机构地区：[1]天津医学院病理学教研室 [2]天津医学院,研究生导师300070

出　　处：《天津医药》1993年第12期707-710,共4页Tianjin Medical Journal

摘　　要：7只5月龄雌性SD大鼠自由饮用80ppm氟化钠(含36ppmF^-)水溶液18周后,加饮20％酒精13周(F+A组)。形态计量表明,于F+A组大鼠:1.肝贮脂细胞的光镜计数较单纯饮用80ppm氟化钠水溶液的大鼠(F组,6只)显著增多,较单纯饮用20％酒精的大鼠(A组,7只)呈增多趋势。2.低倍(2000×)电镜下,较大范围间质内的胶原纤维面积均比F组和A组非常显著地增多。3.较高倍(6600×)电镜下,贮脂细胞周围的胶原纤维面积也均比F组和A组非常显著地增多。结果提示:1.在本实验条件下,氟化钠和酒精的联合饮用,会加重酒精所致大鼠肝间质的纤维化。2.慢性酒精中毒有可能更易于引起高氟区人群,尤其是慢性氟中毒患者肝脏的纤维化。Seven female SD rats of five-month-old freely drank deionic water containing 80ppm sodium fluoride (NaF,corresponding to 36ppm F^-) for 18 weeks, and then took both the NaF solntion and 20％ ethanol for 13 weeks (Group F+A). The results of morphometry showed that in the Group F+A, (1) under light microscopy, the number of Ito cells (N-Ito) was significantly increased as compared with 6 rats drinking 80ppm NaF solution (Group F) only (P<0.01), and tended to be increased with 7 rats drinking 20％ ethanol (Group A) only; (2) under electron microscopy (EM, 2000×), the area of mesenchymal collagen (A-Co Ⅰ) in the livers was signifi cantly increased as compared with both Group F (P<0.01) and Group A (P<0.01); (3) under EM (6000x), the area of collagen around the Ito cells (A-Co Ⅱ) was also significantly increased as compared with both Group F (P<0.01) and Group A(P<0.05). The above morphometric results showed a roughly parallel changes between A-Ito, A-Co Ⅰ, and A-Co Ⅱ. It is clear from the results that sodium fluoride and ethanol certainly induced some combinative injury to the mesenchyma of rat liver in this experiment, and it also suggested that people living in high-fluoride environmeat, especially patients with chronic fluorosis, may be more sensitive to the hepatic damage of chronic alcoholism.

关 键 词：氟中毒 醇中毒 肝纤维化 

分 类 号：R599.9[医药卫生—内科学]