Transformation of insect-resistant gene into cauliflower (Brassica oleracea L.var. botrytis)  

抗虫基因转化花椰菜的研究(英文)

作　　者：吕玲玲 雷建军[2] 宋明[3] 李立云 曹必好[2] 

机构地区：[1]南亚热带作物研究所,广东湛江524091 [2]华南农业大学,广东广州510642 [3]西南农业大学,重庆400716 [4]广东农工商中等专业学校,广东湛江524091

出　　处：《Agricultural Science & Technology》2004年第3期17-21,共5页农业科学与技术（英文版）

摘　　要：Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L-1. The con centration of carbencillin (carb) was 500 mg L-1. 14 transgenic cauliflower pla nts were obtained. The putative transformants were assayed by PCR and Southern b lotting analysis. The results indicated that CpTI gene was transferred into caul iflower successfully.通过根癌农杆菌介导,将抗虫基因-豇豆胰蛋白酶抑制剂(CowpeaTrypsinInhibitor,简称CpTI)基因导入花椰菜无菌苗的下胚轴和子叶中。卡那霉素(Kanamycin,简称Kan)的筛选浓度为15mg/L,抑制农杆菌生长的抗生素选用羧苄青霉素(Carbencillin,简称Carb),浓度为500mg/L。对所获得的14株Kan抗性植株进行PCR扩增,结果显示有8株为阳性;对PCR阳性植株进行Southern分子杂交检测,结果证明CpTI基因已被整合到花椰菜植株的基因组中。

关 键 词：cauliflower (Brassica oleracea L. var. botrytis) Agrobacterium-me diated genetic transformation CpTI gene transgenic plant 

分 类 号：S635.3[农业科学—蔬菜学] S511[农业科学—园艺学]