三甲基品红与脱氧核糖核酸作用的共振光散射光谱的研究  

Determination of Deoxyribonucleic Acid with New Magenta by A Resonance Light-scattering Technique

作　　者：苏界殊[1,2] 陈小明[3] 罗和安[3] 龙云飞[1] 何格梅[1] 

机构地区：[1]湖南科技大学化学化工学院 [2]湘潭大学化学学院,湖南湘潭411201 [3]湘潭大学化学学院

出　　处：《分析测试学报》2005年第1期60-63,共4页Journal of Instrumental Analysis

基　　金：湖南省自然科学基金项目 (00JJY2084) ;湖南省教育厅资助项目 (03C611)

摘　　要：在pH为0.85的三羟甲基氨基甲烷和盐酸 (Tris-HCl)缓冲溶液中 ,三甲基品红(NM)与脱氧核糖核酸(fsDNA,ctDNA)分子作用后共振光光散射增强 ,其强度增加值与DNA的浓度呈线性关系 ,据此 ,建立了一种测定DNA的共振光散射法。该方法线性范围分别为0.02～6.0mg·L -1,0.04～7.0mg·L -1,相关系数分别为0.9984、0.9987 ,检出限分别为18.3、21.8μg·L-1,用于DNA合成样品的测定获得了满意的结果。同时 ,对该反应机理进行了探讨 ,并用紫外Resonance light scattering(RLS)characteristics of the interaction of deoxyribonucleic acid(DNA)with NewMagenta were studied in a buffer of Tris-HCl at pH0.85.The resonance scattering enhancement was found to have a linear relationship with the DNA concentration.A new method for the determination of DNA(fsDNA and ctDNA)at nanogram levels was thus proposed.The linear range for fsDNA is0.02～6.0mg·L -1 ,whereas that for ctDNA is0.04～7.0mg·L -1 .The correlation coefficients are0.9984and0.9987,and the detection limits are18.3and21.8μg·L -1 ,respectively.Effects of interference subˉstances were studied.The method has been satisfactorily applied to the determination of DNAin synthetic samˉples.Furthermore,the reaction mechanism has been studied,and the complex formation constant between NewMagenta and DNA was obtained from the UV-Vis spectra.

关 键 词：三甲基品红 脱氧核糖核酸 共振光散射法 

分 类 号：O657.39[理学—分析化学]