下颌下腺脱细胞基质的制备及其胶原成分观察  

The Development and collagen ingredients analysis of submandibular gland acellular matrix

作　　者：邓春富[1] 谭学新[1] 周青[1] 黄绍辉[1] 徐中飞[1] 王玉新[1] 

机构地区：[1]中国医科大学第一临床学院口腔颌面外科,辽宁沈阳110001

出　　处：《上海口腔医学》2004年第6期528-530,共3页Shanghai Journal of Stomatology

基　　金：辽宁省社会发展基金(2003225007-8)

摘　　要：目的:制备天然的下颌下腺脱细胞基质材料,观察脱细胞后下颌下腺的组织结构,并对其成分进行分析。方法:采用化学除垢剂法对SD大鼠的下颌下腺进行脱细胞处理,通过组织学及免疫组织化学对脱细胞基质材料进行观察及检测。结果:光镜下见下颌下腺细胞消失,基质成分呈筛网状。免疫组织化学证实,在SD大鼠下颌下腺脱细胞前后的组织中,Ⅰ、Ⅱ、Ⅲ型胶原均呈强阳性表达,而Ⅳ、Ⅴ型胶原呈弱阳性或阴性表达。结论:TritonX-100脱细胞处理的大鼠下颌下腺基质中,主要成分为Ⅰ、Ⅱ、Ⅲ型胶原蛋白。PURPOSE: To develop submandibular gland acellular matrix and observe its structure and analyze its histological ingredients. METHODS: The fresh submandibular glands of SD mice were cell-extracted by chemical detergent and histological ingredients were analyzed by immunohistochemistry. RESULTS: Submandibular gland cells disappeared, matrix ingredients appeared network structure under microscopy. Ⅰ,Ⅱ and Ⅲ type collagen proteins of submandibular gland before and after cell-extracted were positive under immunohistochemistry. CONCLUSIONS: The main ingredients of submandibular gland acellular matrix were Ⅰ,Ⅱ and Ⅲ type collagen proteins.

关 键 词：下颌下腺 脱细胞 免疫组化 胶原蛋白 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]