五种水螅基因组DNA的RAPD多态性研究  

GENETIC DNA DIVERSITY OF FIVE SPECIES OF HYDRAS

作　　者：范学铭[1] 肖玮[1] 张彦帅[1] 

机构地区：[1]哈尔滨师范大学生命与环境科学学院生物学系,哈尔滨150080

出　　处：《动物分类学报》2005年第1期205-208,共4页Acta Zootaxonomica Sinica

基　　金：黑龙江省自然科学基金;黑龙江省教育厅自然科学基金资助项目

摘　　要：利用RAPD技术对水螅科 2属 5种水螅进行了基因组DNA多态性研究。 80个随机引物筛选出 1 8个引物 ,共扩增出 30 3个清晰稳定的DNA片段 ,分子量在 0 3～ 3 0kb之间。以UPGMA聚类法构建树状图 ,阐明水螅属种的分类关系 :大庆水螅Hydradaqingensis、北京水螅H beijingensis和强壮水螅H robusta 3种分成两组并聚在一起 ;不同属的漠河水螅H mohensis和绿水螅Chlorohydrasp 聚为一支 ;由此 2属 5种水螅分为两个支系。RAPD (random amplified polymorphic DNA) technique was applied to assess the genetic diversity among five species belonging to two genera of Hydraridae. Eighteen primers were selected from 80 random primers and yielded a total of 303 clear bands ranging from 0.3-3.0?kb. The classification relationships of hydras were clarified using UPGMA. Individuals of the same species clustered respectively first. Hydra daqingensis and H. beijingensis showed close relationship with H. robusta, H. mohensis and Chlorohydra sp. belonging to different genera showed close relationship, so five species of hydras could be divided into two groups.

关 键 词：水螅科 水螅属 属种 基因组DNA多态性 DNA片段 随机引物 RAPD技术 RAPD多态性 UPGMA聚类 树状图 

分 类 号：Q953[生物学—动物学]