防硬膜外粘连膨化聚乙烯复合膜的生物学评价  

Biocompatibility of swelling polyethylene compound biomembrane for preventing pe ridural adhesion

作　　者：戴伯军[1] 叶晓健[1] 吕宏[1] 何海龙[1] 李家顺[1] 贾连顺[1] 

机构地区：[1]解放军第二军医大学长征医院骨科,上海市200003

出　　处：《中国临床康复》2005年第2期66-68,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:对自行研制的医用可吸收粘连材料膨化聚乙烯复合膜的体外生物相容性进行评价。方法:实验于2003-03/2003-08在第二军医大学肝胆外科实验室完成。采用细胞毒性试验、溶血试验和急性全身中毒试验3种方法。结果:①培养的L-929小鼠成纤维细胞经膨化聚乙烯复合膜浸提液处理后形态良好,增值旺盛,膨化聚乙烯复合膜组培养2,4,7dA值分别为0.552±0.044,0.783±0.040,1.198±0.052;阳性对照组分别为0.347±0.025,0.446±0.018,0.565±0.025,二者比较,差异有非常显著性意义(t=26.47～31.12,P<0.01)。细胞相对增殖率≥100%,材料毒性评级为0级。②溶血率为2.21%(<5%),符合溶血试验标准要求。③无急性全身中毒反应。全身急性毒性试验24,48,72h膨化聚乙烯复合膜组动物体质量增加分别为(1.77±0.42),(2.57±0.39),(3.30±0.43)g,阳性对照组分别为-2.79±0.23),(-3.83±0.35),((-3.89±0.34)g,二者比较,差异有非常显著性意义(t=58.34～87.12,P<0.01)。结论:膨化聚乙烯复合膜具有良好的生物相容性。AIM:To evaluate the in vitro biocompatibility of swelling polyethylene compoun d biomembrane,a self made absorbable adhesive material in medicine for the prev ention of epidural adhesion. METHODS:The experiment was completed in the laboratory for Department of Hepat obiliary Surgery,Second Military Medical University of Chinese PLA from March 20 03 to August 2003.The cytotoxicity test,hemolytic test and acute whole body into xication toxicity test were performed with standard method. RESULTS:①The fibroblasts of L 929 mice were in good morphology and prolifera tion after treated with swelling polyethylene compound biomembrance extract solu tion.At 2,4 and 7 days,the absorbance(A) values were 0.552±0.044,0.783±0.040 a nd 1.198±0.052 respectively in polyethylene compound group,significantly differ ent from those in the positive control group(0.347±0.025,0.446±0.018 and 0.565 ±0.025 respectively)(t=26.47 to 31.12,P< 0.01).The relative proliferation rate was higher than 100%,and the toxicity gradation of the material was 0.②The hem olysis rate was 2.21%(< 5%),which was in accordance with the standard of hemol ytic test.③No acute whole body intoxication reaction was found.The body mass of the animals were increased by(1.77±0.42),(2.57±0.39) and(3.30±0.43) g respec tively in polyethylene compound group at 24,48 and 72 hours,and by(-2.79±0.23) ,(-3.83±0.35) and(-3.89±0.34) g in positive control group,so there were sign ificant difference between the two groups(t=58.34 to 87.12,P< 0.01). CONCLUSION:The swelling polyethylene compound biomembrane has good biocompatib ility.

关 键 词：聚乳酸 甲壳素 生物相容性材料 

分 类 号：R318[医药卫生—生物医学工程]