肢体缺血再灌注后关节软骨细胞凋亡的规律  

Regularity of chondrocyte apoptosis after ischemia reperfusion of limbs

作　　者：赵程[1] 王成琪[2] 王继芳[1] 孙新君[2] 张伟旭[2] 

机构地区：[1]解放军总医院骨科,北京市100853 [2]解放军第八九医院骨科研究所,山东省潍坊市260012

出　　处：《中国临床康复》2005年第2期106-108,i005,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察肢体缺血再灌注后关节软骨细胞凋亡情况,探讨其机制及凋亡对关节软骨退行性变的影响。方法:35只成年健康新西兰大白兔随机分为7组(每组5只),行左侧后肢缺血8h(右侧为对照),于再灌注后1,3d,1,2,4,8和12周取膝关节胫骨平台软骨组织,行透射电镜观察,原位末端标记(insituterminaldeoxynucleotidyltransferase-mediateddUTPnickendlabeling,TUNEL)法检测关节软骨细胞凋亡情况。结果:肢体缺血再灌注后1d,实验侧的TUNEL阳性细胞均数犤(19.8±2.1)个/高倍视野)犦明显多于对照侧(P<0.01),3d组最多,达到对照侧的360%,之后逐渐回落。透射电镜下发现缺血再灌注早期大量细胞坏死。结论:肢体缺血再灌注可引起关节软骨细胞损伤,软骨细胞凋亡,导致关节软骨蜕变。AIM:To observe chondrocyte apoptosis after ischemia reperfusion(IR) of limbs, and probe into its mechanism and the influence of apoptosis on the degeneration of articular cartilage. METHODS:Thirty five healthy adult New Zealand white rabbits were randomly div ided into 7 groups with 5 rabbits in each group.After 8 hour ischemia,the left hind limbs were reperfused(right sides as controls) .Then at 1,3 days,1,2,4,8 an d 12 weeks,the grouped rabbits were killed respectively for the excision of arti cular cartilages from the tibia of knee joints.The pathological changes of chond rocytes were observed under transmission electron microscopy(TEM).Apoptotic chon drocytes were detected by in situ terminal deoxynucleotidyl transferase mediate d dUTP nick end labeling(TUNEL). RESULTS:On the first day after IR,the TUNEL positive chondrocytes on the expe rimental sides[(19.8±2.1)pieces/high power sight]was significantly more than th at on the control sides(P< 0.01).On the third day,the number of TUNEL positive chondrocytes reached maximum(360%of control sides).After that,the positive cell s decreased gradually.At the early stage after IR,large amounts of necrosed chon drocytes were discovered under TEM. CONCLUSION:Articular cartilage injury and chondrocyte apoptosis can be induced by IR of limbs,which will lead to degeneration of articular cartilage.

关 键 词：肢体缺血再灌注 关节软骨细胞 细胞凋亡 规律 关节软骨退行性变 

分 类 号：R684[医药卫生—骨科学]