表面脱钙骨基质明胶修复大块骨缺损的成骨作用研究(英文)  

Osteogenesis of surface decalcified bone matrix gelation in the repair of seg mental bone defects

作　　者：蔡贤华[1] 陈庄洪[1] 徐永年[1] 唐运章[1] 刘曦明[1] 徐峰[1] 时宏富[1] 

机构地区：[1]解放军广州军区武汉总医院骨科,湖北省武汉市430070

出　　处：《中国临床康复》2005年第2期228-230,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:大块骨缺损的修复是骨科面临的难题之一,自体骨移植疗效最佳,但来源有限,目前尚缺乏自体骨理想的替代材料。目的:探讨表面脱钙骨基质明胶修复大块骨缺损的疗效及替代自体骨的可能性。设计:实验研究设计应用完全随机设计的对照实验。临床研究设计应用以诊断为依据的前后对照研究。单位:解放军广州军区武汉总医院骨科。对象:实验在解放军广州军区武汉总医院实验室及武汉理工大学测试中心完成。实验材料为32只本地产健康雄性成年家兔,体质量2.5kg,购自湖北省医学科学院。临床研究对象为1991-01/2001-05解放军广州军区武汉总医院骨科收治的大块骨缺损患者31例,男25例,女6例;平均年龄9岁。方法:将32只家兔随机分成A,B两组,两组双侧桡骨制成1cm骨缺损,两组左侧植入表面脱钙骨基质明胶surface-demineralizedbonematrix()(gelatin,SDBMG,A组右侧植入全脱钙骨基质明胶whollydemineralizedbonematrixgelatin,WDBMG,B组右侧植入自体骨。临床采用人SDBMG)治疗大块骨缺损31例。主要观察指标:①动物术后定期进行X射线、组织学检查(A组)及生物力学测定(B组)。②术后定期进行X射线检查。结果:SDBMG呈由表及里的“渐进”诱导成骨过程;SDBMG的骨修复作用与自体骨相近,抗压强度与自体骨侧的差异无显著性意义;临床随访?BACKGROUND:Repair of segmental bone defects is one of the difficult problems i n orthopaedics.Although the therapeutic effect on bone autograft is the best,the source is limited and there is lack of suitable substitutive materials for auto logous bone. OBJECTIVE:To probe into the therapeutic effects of surface decalcified bone m atrix gelation(SDBMG) and the substitutive possibility of autologous bone on rep air of segmental bone defects. DESIGN:The research was designed as completely randomized controlled experimen t.The clinical research was designed as pre and post controlled study on the b asis of diagnosis. SEETING:Department of Orthopaedics,Wuhan General Hospital of Guangzhou Militar y Area Command. MATERIALS and PARTICIPANTS:The experiment was accomplished in Laboratory of Wu han General Hospital of Guangzhou Military Area Command,and Testing Center of Wu han University of Technology.Thirty two local healthy adult male rabbits were e mployed as the materials in the experiment,2.5 kg in body mass,bought from Hubei Academy of Medical Sciences.The clinical subjects were 31 patients(male 25 and female 6,a mean age of 9 years old) with segmental bone defects hospitalized in the Department of Orthopaedics,Wuhan General Hospital of Guangzhou Military Area Command during January 1991 to May 2001. METHODS:The 32 rabbits were randomized into group A and group B.The bilateral radial bones were prepared into 1 cm bone defect in both groups.In both groups, SDBMG was grafted on the left side;in Group A,wholly decalcified bone matrix ge latin(WDBMG) was grafted on the right side;and in Group B,the autologous bone wa s grafted on the right side.Human SDBMG was provided to treat 31 cases of segmen tal bone defects. MAIN OUTCOME MEASURES:①Regular X ray and histological examinations after ope ration in animals(Group A) and biomechanical determination(Group B ).②Regular X ray examinations after operation. RESULTS:SDBMG induced osteogenesis process “gradually' from exterior to inter ior.The bone rep

关 键 词：骨基质 明胶 骨/损伤 骨生成 

分 类 号：R641[医药卫生—外科学]