Ⅱ型胶原海绵填充材料修复兔关节软骨缺损(英文)  

Repair of articular cartilage defect in rabbit with type Ⅱ collagen sponge filling material

作　　者：叶惠贞[1] 李斯明[1] 叶春婷[1] 杨小红[1] 黄雪芳[1] 梁佩红[1] 

机构地区：[1]广州市红十字会医院,暨南大学第四附属医院,广州市创伤外科研究所,广东省广州市510220

出　　处：《中国临床康复》2005年第2期231-233,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:修复关节软骨缺损一直是骨科医师致力解决的难题,以前采用自体软骨膜、骨膜或异体骨软骨片移植,但存在供体来源有限、固定困难,以及出现软骨内骨化、软骨下骨与修复性软骨的分层现象等。Ⅱ型胶原是软骨基质的主要成分,对关节软骨缺损的修复应有一定的作用。目的:探讨Ⅱ型胶原海绵对修复关节软骨缺损的效果。设计:随机对照的实验研究。地点和材料:实验地点为广州市创伤外科研究所。材料:普通级成年雄性纯种新西兰兔24只48膝,体质量(2.29±0.25)kg,标准饲料分笼喂养。干预:在股骨滑车面钻孔为直径5mm、深3mm的全层关节软骨缺损,按随机数分为填充组(左膝关节缺损部位植入Ⅱ型胶原海绵)和对照组(右膝关节缺损部位作为空白对照)。主要观察指标:术后12周内,每双数周对缺损修复情况行大体形态和组织学观察。结果:10～12周,对照组:缺损区由白色、质软、按压无阻抗的组织修复,修复组织仍低于周围关节面,边界仍清晰可辨,组织学以类似炎症反应的机制修复缺损,最终以透明变性的纤维组织的增生来填补缺损部位;填充组:缺损区由半透明状、质韧光滑有光泽,按压有阻抗并有弹性的组织修复,修复组织与周围软骨外形上已基本相似,不易区分,组织学未见有炎症反应的过程,内骨组织和软骨组织增生活跃,并可见大量类骨组织和?BACKGROUND:The repair of articular cartilage defect is always a problem that i s dedicatedly solved by doctors of orthopaedics.Autologous perichondrium,periost eum or allografting of cartilage have been used previously;however,the source of the donor is limited,the fixation is difficult as well as the occurrence of end ochondrial ossification and delamination between the inferior cartilage and repa rative cartilage,etc.Type Ⅱcollagen,the main component of cartilage matrix,has certain effects in the repair of articular cartilage defect. OBJECTIVE:To investigate the effects of type Ⅱcollagen sponge filling on the repair of articular cartilage defect. DESIGN:A randomized controlled trial. SETTING and MATERIALS:The study was conducted in the Guangzhou Institute of Tr aumatic Surgery.Materials were 24 adult male purebred New Zealand Rabbits(48 kne es),ordinary grade,with a body mass of (2.29±0.25) kg.Animals were fed with sta ndard feeding in separate cage. INTERVENTIONS:A full thickness defect in articular cartilage was made on the femoral trochlear surface by a drill of 5 mm in diameter and 3 mm in depth.Rabbi ts were allocated into filling group(type Ⅱcollagen sponge was grafted into lef t keen joint defect) and control group(right knee joint defect site was set as c ontrol) according to random number table. MAIN OUTCOME MEASURES:Gross morphological and histological observation of the defect repair in each dual week within 12 weeks after operation. RESULTS:During 10-12 weeks,in control group:The defect area was repaired by w hite and soft tissue that had no resistance to press.The repaired tissue was sti ll lower than the surrounding articular surface with clear boundary.By histologi cal observation,it was found that the defect was repaired by the mechanism simil ar to inflammatory reaction and the defect is ultimately filled by the hyperplas ia of hyaline degenerative fibrous tissues.In filling group:the defect was repai red by semi transparent,smooth,textured tissues with polish that had resis

关 键 词：软骨 关节 生物医学工程 生物相容性材料 

分 类 号：R318[医药卫生—生物医学工程]