间歇和连续热化疗对兔肝VX-2移植瘤模型的安全有效性探讨(英文)  

Safety and effectiveness of intermittent and continuous thermochemotherapy on VX-2 transplanted tumor model in rabbit liver

作　　者：张洪新[1] 刘燕[2] 魏娟[1] 刘毅勇[1] 曹玮[1] 倪代会[1] 李文献[1] 韩瑞炀[1] 

机构地区：[1]第四军医大学唐都医院介入放射科,陕西省西安市12710038 [2]第四军医大学唐都医院眼科,陕西省西安市12710038

出　　处：《中国临床康复》2005年第2期233-235,共3页Chinese Journal of Clinical Rehabilitation

基　　金：全军"十五"医学青年基金(01Q124);第四军医大学科技创新工程基金(CX99A016);陕西省社发攻关课题(2003K10G64)~~

摘　　要：目的:通过兔肝VX-2移植瘤模型,比较阿霉素间歇性热灌注与连续性热灌注2种方法对兔呼吸、心率、体温及VX-2肿瘤内阿霉素浓度的影响,验证间歇性热灌注的有效性和安全性。方法:在30只新西兰大白兔后腿上建立VX-2肿瘤模型,并随机分为1,2,3组(随机分组见正文,每组10只。经股动脉插管,计算机数字减影)(DigitalSubtractionAngiography,DSA造影证实为肿瘤供血动脉后,分别)给予常温100mL盐水加阿霉素(ADM)灌注、60℃热盐水100mL加阿霉素连续灌注、60℃热盐水100mL加阿霉素间歇性灌注。灌注过程中,测量第2,3组60℃热灌注组肿瘤组织内43～45℃持续时间;灌注后即时检测各组兔呼吸次/分、心率次/分)、体温℃及肿瘤组织内()(()阿霉素浓度。结果:常温灌注组阿霉素质量浓度为(7.1±2.2)mg/L,60℃连续灌注组为(17.2±1.6)mg/L,60℃间歇性灌注组为(16.5±3.4)mg/L。3组间阿霉素质量浓度有显著差异F=48.95,P=0.000),60℃间歇灌(注组阿霉素质量浓度与60℃连续灌注组差异无显著性(P>0.05);60℃间歇性灌注组43～45℃持续时间为24.3±2.4)min,60℃连续(灌注组为22.5±1.4)min,60℃间歇性灌注组43～45℃持续时间与(60℃连续灌注组差异无显著性意义(F=4.20,P>0.05)。3组间呼吸、心率、体温差异有显著性意义(F值分别为14.58,33.07,AIM:To compare the impacts of adriamycin(ADM) intermittent and continuous heat infusion on respiration,heart rate and temperature of the rabbit as well as the concentration of ADM in VX 2 tumor for the verification of the safety and effe ctiveness of intermittent heat infusion through VX 2 transplanted tumor model i n rabbit liver. METHODS:VX 2 tumor models were established respectively in the thighs of 30 N ew Zealand rabbits.All 30 rabbits were randomly allocated into three groups with 10 rabbits each.After catheterizing into femoral artery,which was demonstrated as the supply artery of tumors by digital subtraction angiography(DSA),animals i n three groups were infused with 100 mL saline at normal temperature and ADM,100 mL saline at 60 ℃and ADM continuously and 100 mL saline at 60 ℃and ADM interm ittently respectively.During infusion,the 43 ℃to 45 ℃lasting time in the tumor tissues of the two 60 ℃groups was measured.After infusion,the respiration(time /minute),heart rate(beats/minute) and temperature(℃)of the rabbits in each grou p as well as the concentrations of ADM in tumor tissue were measured immediately . RESULTS:The concentration of ADM was (7.1±2.2) mg/L in normal temperature inf usion group,(17.2±1.6) mg/L in 60 ℃continuous infusion group and(16.5±3.4) in 60 ℃intermittent infusion group.There were significant differences among three groups(F=48.95,P=0.000),but there was no significant difference between 60 ℃in termittent infusion group and 60 ℃continuous infusion group(P >0.05).43℃to 45 ℃lasting time was(22.5±1.4) minutes in 60 ℃continuous infusion and (24.3±2.4 ) minutes in 60 ℃intermittent infusion group(F=4.20,P >0.05).There were signifi cant differences among three groups in respiration,heart rate and temperature(F= 14.58,33.07,10.00,P< 0.01),but there was no significant difference in the respir ation,heart rate and temperature between 60 ℃intermittent infusion and normal t emperature infusion group(P >0.05). CONCLUSION:Intermittent thermochemothe

关 键 词：灌注 局部 药物疗法 联合 动脉 兔 阿霉素 

分 类 号：R318[医药卫生—生物医学工程]