超级杂交水稻两优培九光抑制差异表达基因的遗传来源  

Genetic Origins of Differentially Expressed Genes Under Photoinhibition Stress in Super Hybrid Rice Liangyoupeijiu

作　　者：姜国强 郭晋隆 孟庆伟[2] 陈由强[3] 王洪新[1] 胡志昂[1] 匡廷云[1] 

机构地区：[1]中国科学院植物研究所光合作用与环境分子生理学重点实验室/资源植物分子与发育生物学研究中心,北京100093 [2]山东农业大学生命科学学院,泰安271018 [3]福建师范大学生物工程学院,福州350007

出　　处：《中国农业科学》2005年第1期33-37,共5页Scientia Agricultura Sinica

基　　金：国家重点基础研究发展规划项目(G199801001)

摘　　要：利用改进的银染mRNA差异显示技术研究杂种超级杂交水稻两优培九及其母本培矮64S和父本9311在光抑制胁迫条件下基因表达的变化,共得到了杂种的167个差异表达基因片段。对这些差异片段进行分类和遗传来源分析表明,杂种光抑制差异表达基因多数为父母本所共有,是母本和父本共同遗传的结果。少数基因表现为母本或父本的单亲特异性,或无明显亲本来源,说明某种未知机制可能参与了光抑制相关基因从亲本到杂种的遗传过程。The mRNA differential display visualized by silver staining was applied to study genes expressed differentially under stress condition of photoinhibition in super hybrid rice Liangyoupeijiu and its female parent Peiai 64S and male parent 9311. One hundred and sixty-seven differentially displayed cDNA fragments of hybrid rice were obtained and their genetic origins were studied. The results suggested that most of the differentially expressed genes were inherited from both male and female parents rather than either of them because they existed in both male and female parents. Some of the differentially expressed gene fragments seemed to originate from a single-parent or from none of the parents, suggesting the existence of some unclear mechanisms.

关 键 词：杂种 超级杂交水稻 母本 父本 光抑制 两优培九 遗传 差异表达基因 相关基因 基因表达 

分 类 号：S511[农业科学—作物学] S513