抑制素α亚基在大鼠下丘脑与垂体的免疫组织化学定位  

Immunohistochemical Localization of Inhibin á Subunit in Hypothalamus and Pituitary of Rat

作　　者：王根林[1] 柳伟荣[1] 李莲[1] 蔡亚非[1] 闫玉琴[1] 张利军[1] 

机构地区：[1]南京农业大学动物科技学院,南京210095

出　　处：《中国农业科学》2005年第1期171-175,共5页Scientia Agricultura Sinica

基　　金：教育部博士基金资助项目(20010307007)

摘　　要：用链酶亲和素-生物素过氧化物酶复合物(SABC)免疫组织化学方法,使用抑制素α亚基单克隆抗体对注射抑制素α片段及抑制素粗提物后的去卵巢大鼠的下丘脑和垂体进行了免疫组织化学定位。结果表明,在下丘脑与垂体存在抑制素α亚基单克隆抗体的特异性阳性反应位点,并存在剂量与时间上的依赖关系,说明抑制素α亚基可以通过血脑屏障到达下丘脑和垂体,为探讨抑制素α亚基在血脑屏障的转运机制提供了形态学上的依据。Using the Strept Avidin Biotin-peroxidase Complex(SABC) immunohistochemical staining technique, immuno- histochemical localization of inhibin á subunit of rat hypothalamus and pituitary with inhibin á subunit monoclonal antibody was performed at different times post the injection. The sexually matured ovari-ectomized rats were injected with inhibin á subunit crude abstracts and different levels of inhibin á subunit fragments. The results showed that positive reaction sites in dose and time dependant ways were detected in the hypothalamus and pituitary, suggesting that inhibin á subunit could pass through the blood-brain barrier arriving at hypothalamus and pituitary. It provided new morphological basis for the research field of a passage mechanism of the subunit through blood-brain barrier.

关 键 词：垂体 下丘脑 抑制素 血脑屏障 大鼠 单克隆抗体 Α亚基 免疫组织化学定位 位点 过氧化物酶 

分 类 号：S852.4[农业科学—基础兽医学]