rhTGF-β1、rhBMP-2和rhbFGF单独或者联合应用对BMSC的ALP活性和钙化能力影响  

The effects of basic fibroblast growth factor, transforming growth factor-β1 and bone morphogenetic protein-2 on the alkaline phosphate and calcification cap acity of rat bone marrow stromal cells

作　　者：刘宏伟[1] 李才良[2] 

机构地区：[1]广州第一军医大学南方医院口腔科,510515 [2]武警广东总队医院口腔科

出　　处：《实用口腔医学杂志》2005年第1期31-34,共4页Journal of Practical Stomatology

基　　金：广东省科技计划项目课题资助 (项目编号C30 70 4 )

摘　　要：目的 :研究重组人碱性成纤维细胞生长因子 (rhbFGF)、重组人转化生长因子 β1(rhTGF β1)和重组人骨形成蛋白 2 (rhBMP 2 )单独和联合使用 ,对骨髓基质细胞 (BMSC)碱性磷酸酶 (ALP)活性和钙化能力影响。方法 :体外培养大鼠骨髓基质细胞 ,分别用一定浓度的rhTGF β1(5 μg/L)、rhBMP 2 (2 0 0 μg/L)和rhbFGF(1μg/L)单独或者联合应用 ,测定第 12、14天的碱性磷酸酶活性 ;加入矿化诱导液 ,用Vonkossa染色 ,检测 10、2 0和3 0天的钙化情况。结果 :rhBMP 2可增强BMSC的ALP活性 ,rhbFGF和rhTGF β12种因子单独或者联合使用抑制BMSC的ALP活性 ,rhTGF β1和rhBMP 2以及rhbFGF和rhBMP 2联合使用时 ,抑制BMSC的ALP活性 ;3种生长因子联合使用时 ,抑制BMSC的ALP活性 ,研究发现f +b组和b组可显著促进BMSC向成骨细胞转化 ,增强细胞的矿化能力 ;其它各组对BMSC向成骨细胞转化、矿化能力无显著作用。结论 :生长因子rhBMP 2(2 0 0 μg/L)单独应用和rhbFGF(1μg/L)在此浓度下结合使用可以提高体外培养骨髓基质细胞的钙化能力。Objective: To study the effects of basic fibroblast growt h factor(rhbFGF), transforming growth factor-β1(rhTGF-β1) and bone morphogenet ic protein-2(rhBMP-2) on the alkaline phosphate and calcification capacity of rat bone marrow stromal cells(BMSCs) in vitro. Methods: Rat BMSCs were cultured in DMEM with the addition of rhbFGF(1 μg/L), rhTGF-β1(5 μg/L) and rhBMP-2(200 μg/L) alone or in combination.Alkaline phosphoric activ ity of the cells was mesured after 12- and 14-day culture respectively. For th e test of calcification, BMSCs were cultured in minerialization inducing medium with the addtion of above mentioned cytokines, calcification capacity of the cel ls was examined with Vonkossa staining after 10,20 and 30 days culture respectiv ely.Results: rhBMP-2 strengthened the ALP activity, rhbFGF, rh TGF-β 1 alone or in combination inhibited ALP activity, rhTGF-β 1 combined w ith rhBMP-2 and rhbFGF combined with rhBMP-2 inhibited ALP activity;the 3 kind s of growth factor in combination inhibited ALP activity. Vonkossa staining reve aled that rhBMP-2 alone or combined with rhbFGF increased the calcification of BMCs(P<0.05).Conclusion: rhBMP-2 alone or combined with rhb FGF can increase the calcification of the marrow stromal cells.

关 键 词：骨髓基质细胞 碱性成纤维细胞生长因子 转化生长因子β1 骨形成蛋白-2 碱性磷酸酶 钙化能力 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]