应用分子梳技术对DNA与组蛋白相互作用的研究  

Study of interactions between DNA and histone with molecular combing method

作　　者：刘玉颖[1] 窦硕星[1] 王鹏业[1] 谢平[1] 王渭池[1] 

机构地区：[1]中国科学院物理研究所软物质物理实验室,北京100080

出　　处：《物理学报》2005年第2期622-627,共6页Acta Physica Sinica

基　　金：国家自然科学基金 (批准号 :60 0 2 5 5 16和 10 3 3 410 0 )资助的课题~~

摘　　要：利用分子梳技术对λ DNA和组蛋白的相互作用进行了研究 .通过这种简单有效的方法 ,我们将λ DNA分子拉伸到 2 6— 2 8μm ,相当于其原长 (约 16 2 μm)的 1 6— 1 7倍 .当组蛋白与DNA结合后 ,DNA分子发生凝聚现象 ,复合体的拉伸长度明显变短 ,其峰值分布在 10— 14 μm之间 .DNA 组蛋白复合体的拉伸长度与组蛋白的浓度、与碱基对和荧光染料的比例有显著的关系 .The interactions between DNA and histone are studied by using the molecular combing method. With this simple but effective method, X-DNA molecules are stretched to a length of 26-28 part, 1.6-1.7 times of their contour length (similar to 16.2 mum). After DNA binding of histones, DNA molecules are condensed and the lengths of the stretched complexes become significantly shorter, with length distribution peak lying between 10-14 mum. The lengths of the stretched DNA-histone complexes are closely related to the histone concentration and the mixing ratio of DNA base pair and dye molecule.

关 键 词：组蛋白 分子梳技术 DNA分子 DNA结合 碱基对 相互作用 复合体 荧光染料 研究 关系 

分 类 号：Q503[生物学—生物化学]