复方鱼腥草片的鉴别和含量测定  

Identification and quantitative determination of Fufang Yuxingcao tablets

作　　者：车晓彦[1] 贺亚玲[1] 尹靖先[1] 邓晓鸿[1] 刘军[1] 

机构地区：[1]四川省药品检验所,四川成都610036

出　　处：《华西药学杂志》2005年第1期65-67,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的　建立复方鱼腥草片中鱼腥草的鉴别和黄芩苷的含量测定方法。方法　采用薄层色谱法和高效液相色谱法。结果　鱼腥草的薄层色谱分离良好 ,阴性样品无干扰 ;用HPLC法测定黄芩苷的含量 ,其线性范围为 0 .0 5 3～ 3.36 8μg (r =0 .9999) ,平均回收率 98.4 3% ,RSD =1.96 % (n =5 )。结论　所用方法简便、灵敏、准确 ,可用于复方鱼腥草片的质量控制。OBJECTIVE To establish an identification method of Herba Houttuyniae and a quantitative determination method of baicalin contents in Fufang Yuxingcao tablets.METHODS TLC and HPLC were used. RESULTS The TLC method could identify Herba Houttuyniae and HPLC method could quantitatively determine baicalin contents in Fufang Yuxingcao tablets . The linear range was 0.053-3.368 μg (r=0.9999).The average recovery was 98.43% with RSD of 1.96%. CONCLUSION These methods are simple,sensitive and accurate.It can be used to control the quality of Fufang Yuxingcao tablets.

关 键 词：复方鱼腥草片 鉴别 黄芩苷 含量测定方法 阴性 HPLC法 薄层色谱法 平均回收率 分离 样品 

分 类 号：R286.0[医药卫生—中药学] R927.2[医药卫生—中医学]