氯沙坦和卡托普利对大白兔心肌缺血再灌注损伤影响的比较  

Comparison of effects of losartan and captopril on myocardial ischemia-reperfusion injury in rabbits

作　　者：陈剑峰[1] 曾秋棠[2] 

机构地区：[1]武警湖北总队医院四内科,武汉430061 [2]华中科技大学同济医学院附属协和医院心内科,武汉430022

出　　处：《武警医学》2005年第1期30-33,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的　比较氯沙坦和卡托普利对心肌缺血再灌注损伤的影响。方法　健康大白兔随机分为 2组 ,每组各 10只 :(1)对照组 :每日灌服卡托普利 4 0mg/kg ;(2 )治疗组 :每日灌服氯沙坦 4 0mg/kg,给药 7d。所有大白兔制作心肌缺血再灌注动物模型 ,观察心律失常的发生率、心率的变化。复灌后 12 0min采血 ,测定血清肌酸磷酸激酶 (CPK)、乳酸脱氢酶 (LDH)、一氧化氮 (NO)、一氧化氮合酶 (eNOS)、血管紧张素II(AngII)、丙二醛 (MDA)及超氧化物歧化酶 (SOD)的含量。实验完毕 ,分别用双重染色确定心肌梗死的面积。结果　氯沙坦治疗组CPK、LDH和MDA较对照组明显降低 (P <0 .0 5 ) ,而NO、eNOS和SOD则明显增高 ,降低了心肌缺血再灌注诱发室性心律失常的发生率 ,具有明显的负性频率作用。治疗组梗死心肌占危险区心肌重量百分比 (30 .2 1± 5 .10 ) % ,较对照组 (37.6 7± 6 .4 6 ) %显著降低。结论　氯沙坦能明显减轻心肌缺血再灌注损伤。Objective To investigate the effects of losartan and captopril on myocardial ischemia-reperfusion injury in rabbit model.Methods Twenty healthy rabbits were randomly divided into two groups:the control group was gavaged with captopril 40 mg/kg and the treatment group was gavaged with losartan 40 mg/kg at fixed time per day. At the end of one week,all rabbits were subjected to a sustained 30 min of left anterior desending coronary arterial occlusion and 120 min reperfusion. After 60 min reperfusion, blood samples were obtained for determination of serum creatine phosphokinase(CPK), malodialdehyde (MDA),latic dehydrogenase (LDH),nitric oxide synthase (eNOS), nitrogen monoxide(NO),and angiotensin Ⅱ(AngⅡ) from the right common carotid artery .In the whole experimental process, cardiogram was monitored synchronously by limb lead Ⅰ to observe the incidence of the ventricular arrhythmia and the heart rate.After experiment ,the rabbits were sacrificed and the hearts were taken quickly.Infarction sizes in heart were determined by double staining.Results Infarction size as a ratio of weight of infarcted tissue to weight at risk was significantly smaller in losartan group (30.21±5.1%) than in control group (37.67± 6.46%)(P< 0.01).The values of serum CPK,LDH,and MDA during reperfusion in losartan group were significantly lower than in control group(P<0.05),and the values of serum SOD,NO,and eNOS were significantly higher(P<0.05).The incidence and duration of ventricular arrhythmia induced by myocardial ischemia-reperfusion were significantly more decreased in losartan group than in control group.Conclusion Losartan can alleviate myocardial reperfusion injury.

关 键 词：氯沙坦 心肌缺血再灌注损伤 大白兔 卡托普利 对照组 治疗组 NOS 结论 乳酸脱氢酶(LDH) 面积 

分 类 号：R541[医药卫生—心血管疾病] R544[医药卫生—内科学]